The objective of this project is to elucidate at the molecular level how force-generating microtubule motor proteins transport vesicles and mitochondria in neurons and how specific defects in those transport mechanisms cause the length-dependent axon degeneration that underlies human motor neuron diseases. Tremendous progress has been made by in vitro studies of how microtubule motor proteins generate force, how they walk progressively along microtubules, and how they can be autoregulated by switching between two states of folding. In contrast, relatively little is known about how such in vitro capabilities are used to accomplish the many intricately controlled transport processes that are essential for the growth and specialized functions of living cells. We have developed a powerful in vivo experimental model system that will allow rigorous investigation at the molecular level of how motor proteins function specifically in the transport o single organelles in neurons of living animals. Our system enables us to study the influences of any desired change in a motor protein's sequence on the detailed transport behavior of mitochondria and other types of organelles in Drosophila. It was with Drosophila that we made the original discovery that defects in certain microtubule motors cause the length-dependent axon degeneration and progressive distal paralysis that in humans underlie motor neuron diseases. We have just completed development of a new approach that allows homologous gene replacement at the kinesin heavy chain (Khc) microtubule motor locus in Drosophila. Using that new approach, along with standard non-homologous gene insertion, time-lapse microscopy of fluorescently tagged organelles, genetics, and biochemistry, we will build on our past work to determine in vivo: 1) how the C-terminal tail of Khc contacts its N-terminal head for autoregulatory control of movement, and how that control contributes to axonal organelle transport;2) how the functions of three different types of microtubule motors, including ones that transport cargoes in opposite directions, are highly interdependent (i.e., loss of any one type of motor inhibits the function of the others);and 3) how defective transport of mitochondria may specifically trigger the sort of length-dependent axon degeneration that causes motor neuron diseases. It is anticipated that the results of these studies will generate substantial advances fo understanding fundamental mechanisms of directed organelle transport, and will lead to the identification of targets within those mechanisms for the development of therapeutic treatment of neurodegenerative disease.
This project will investigate molecular mechanisms of long-distance transport of vesicles and mitochondria in the axons of neurons and how defects in specific transport mechanisms cause human motor neuron disease. The experimental system used, Drosophila, is powerful and economical, and will allow studies of transport mechanisms at the molecular level in unperturbed axons in living animals. The expected results will advance the understanding of in vivo transport mechanisms and of the pathology of neurodegeneration.
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|Djagaeva, Inna; Rose, Debra J; Lim, Angeline et al. (2012) Three routes to suppression of the neurodegenerative phenotypes caused by kinesin heavy chain mutations. Genetics 192:173-83|
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|Macias, Hector; Moran, Angel; Samara, Yazeed et al. (2011) SLIT/ROBO1 signaling suppresses mammary branching morphogenesis by limiting basal cell number. Dev Cell 20:827-40|
|Albertson, Roger; Cao, Jian; Hsieh, Tao-shih et al. (2008) Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle. J Cell Biol 181:777-90|
|Barkus, Rosemarie V; Klyachko, Olga; Horiuchi, Dai et al. (2008) Identification of an axonal kinesin-3 motor for fast anterograde vesicle transport that facilitates retrograde transport of neuropeptides. Mol Biol Cell 19:274-83|
|Saunders, Adam M; Powers, James; Strome, Susan et al. (2007) Kinesin-5 acts as a brake in anaphase spindle elongation. Curr Biol 17:R453-4|
|Horiuchi, Dai; Collins, Catherine A; Bhat, Pavan et al. (2007) Control of a kinesin-cargo linkage mechanism by JNK pathway kinases. Curr Biol 17:1313-7|
|Haghnia, Marjan; Cavalli, Valeria; Shah, Sameer B et al. (2007) Dynactin is required for coordinated bidirectional motility, but not for dynein membrane attachment. Mol Biol Cell 18:2081-9|
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