The incidence of obesity has increased dramatically over the last fifty years. Over half of all Americans are over-weight and over one third are classified as clinically obese. The health ramifications of this epidemic of obesity are seen in th concomitant increase in the incidence of type 2 diabetes. An estimated 25 million Americans have diabetes with 95% of those being type 2. The most commonly prescribed therapeutic drugs used for type 2 diabetes target the AMP-activated protein kinase (AMPK). Therefore, understanding how AMPK is normally regulated is of great significance to human health. This proposal uses baker's yeast as its model system to study the regulation of the yeast AMPK. In both yeast and human cells, the overall structure of AMPK as well as its modes of regulation is highly conserved. The speed and synergy of genetic and biochemical studies in yeast make this an ideal system to dissect the regulation of AMPK. Past studies from our lab have shown that the activation of Snf1, the yeast name for AMPK, involves at least two steps: phosphorylation of the Snf1 activation loop and a second activation step mediated by the beta and gamma regulatory subunits of the Snf1 complex. We have shown that the first step, control of activation loop phosphorylation, is not regulated at the level of phosphate addition. Surprisingly, it is the dephosphorylation step that responds to cellular energy status. In recent studies we have been able to recapitulate the regulated dephosphorylation of Snf1 kinase in vitro in a purified system. Under conditions of energy stress, Snf1 is primarily in the active phosphorylated state. We can show that binding to low energy adenylate ligands promotes the formation of a phosphatase resistant conformation, thus stabilizing the active form of Snf1.
Specific aim 1 of this proposal will characterize the molecular mechanism by which adenylate ligands promote the formation of the phosphatase resistant state using purified Snf1 enzymes. In other studies, we have shown that the ligand-mediated protection from dephosphorylation is only one level of regulation. Additional means of regulation must also be operating.
In Specific aim 2, we will use genetic and biochemical screens to identify additional components and signaling pathways that regulate Snf1 kinase activity in vivo. In the final aim we examine the mechanism by which the phosphatase which inactivates Snf1 is targeted to specific substrates. In all, these studies will further our understanding of the regulation of AMPK and will help to develop new and more effective interventions to treat diabetes.

Public Health Relevance

The dramatic increase in the incidence of obesity in the United States has produced a concomitant increase in the incidence of type 2 diabetes. The most widely prescribed drug used to treat type 2 diabetes targets a protein known as AMP-activated protein kinase. My lab studies how this important protein controls cellular metabolism in a normal cell and seeks to understand how changes in glucose levels regulate its activity.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM046443-21A1
Application #
8500616
Study Section
Cellular Signaling and Regulatory Systems Study Section (CSRS)
Program Officer
Maas, Stefan
Project Start
1991-07-01
Project End
2017-02-28
Budget Start
2013-04-01
Budget End
2014-02-28
Support Year
21
Fiscal Year
2013
Total Cost
$362,188
Indirect Cost
$124,688
Name
University of Pittsburgh
Department
Genetics
Type
Schools of Medicine
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Schmidt, Martin C (2013) Signaling crosstalk: integrating nutrient availability and sex. Sci Signal 6:pe28
Mayer, Faith V; Heath, Richard; Underwood, Elizabeth et al. (2011) ADP regulates SNF1, the Saccharomyces cerevisiae homolog of AMP-activated protein kinase. Cell Metab 14:707-14
Zhang, Yuxun; McCartney, Rhonda R; Chandrashekarappa, Dakshayini G et al. (2011) Reg1 protein regulates phosphorylation of all three Snf1 isoforms but preferentially associates with the Gal83 isoform. Eukaryot Cell 10:1628-36