Transcription is a pivotal point when cells control gene expression. Until recently, it was thought that the control of transcription in animal cells occurred primarily during formation of a preinitiation complex. The hsp70 gene of Drosophila was an apparent outlier and served as a paradigm for a small number of genes that regulated transcription after initiation by controlling the duration of a pause, which occurs in the promoter proximal region. However, through the use of new genomic techniques, genome-wide mapping of Pol II has revealed that thousands of genes in metazoans have Pol II paused in a region 20 to 50 nucleotides downstream from the transcription start. Numerous studies have documented the presence of Pol II at promoters in cells, but few have endeavored to investigate the mechanisms that regulate the pause. The overall goal of this A1 renewal application is to elucidate mechanisms that regulate promoter proximal pausing using Drosophila as a model system. We have reconstituted promoter proximal pausing in Drosophila nuclear extracts and this will provide a biochemical basis for investigating pausing. Also, we have developed a new technique called permanganate- ChIP-seq that will allow us to monitor the behavior of paused Pol II genome wide in response to various experimental conditions designed to probe mechanisms of pausing in vivo. The proteins, DSIF and NELF, have central roles in pausing and somehow cause Pol II to pause upon associating with the elongation complex. An enigmatic component of Pol II constituting the carboxyl terminal domain (CTD) of the largest subunit also somehow regulates pausing. Finally, our genomic analyses of paused Pol II has provided strong evidence for at least two distinct mechanisms of pausing. One mechanism is orchestrated by a DNA binding protein called GAGA factor. GAGA factor recruits NELF to promoters to facilitate binding of NELF to the elongation complex. Another mechanism is orchestrated by a DNA binding protein we call M1BP. M1BP- associated genes have strikingly ordered arrays of nucleosomes in their gene bodies, and our analyses indicate that pausing results from a collision between Pol II and the first nucleosome. To obtain insight into mechanisms of promoter proximal pausing, we aim to do the following: 1) Structure/function analyses of DSIF and NELF; 2) Mutational analysis of the Pol II CTD; 3) Functional analysis of M1BP-mediated transcription. The analyses of DSIF and NELF focuses on the roles of RNA binding domains in DSIF and NELF and a small region of DSIF that we found contributes to pausing. The analyses of the Pol II CTD explores the possibility that distinct regions of the CTD have specific functions in pausing. The functional analysis of M1BP focuses on our new discovery that M1BP binds a glutathione S-transferase and this glutathione S-transferase can act at a gene's promoter. The combination of biochemical and in vivo approaches that can be applied to Drosophila uniquely qualifies this project for obtaining significant insight into the mechanism of promoter proximal pausing, thereby having significant impact our fundamental understanding of gene regulation.

Public Health Relevance

Transcriptional regulation plays a prominent role in the appropriate expression of genes, and many diseases arise because of defects in gene expression. Recent research has revealed that a mechanism of transcriptional regulation called promoter proximal pausing, once thought to occur at only a handful of genes, actually happens at thousands of genes including ones involved in development and stem cell renewal. Promoter proximal pausing will be studied in the model organism, Drosophila, which offers a unique combination of experimental approaches for understanding this important biological process.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM047477-24
Application #
9406130
Study Section
Molecular Genetics B Study Section (MGB)
Program Officer
Sledjeski, Darren D
Project Start
1992-05-01
Project End
2018-12-31
Budget Start
2018-01-01
Budget End
2018-12-31
Support Year
24
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Pennsylvania State University
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
003403953
City
University Park
State
PA
Country
United States
Zip Code
16802
Portz, Bede; Lu, Feiyue; Gibbs, Eric B et al. (2017) Structural heterogeneity in the intrinsically disordered RNA polymerase II C-terminal domain. Nat Commun 8:15231
Qiu, Yijun; Gilmour, David S (2017) Identification of Regions in the Spt5 Subunit of DRB Sensitivity-inducing Factor (DSIF) That Are Involved in Promoter-proximal Pausing. J Biol Chem 292:5555-5570
Mayfield, Joshua E; Robinson, Michelle R; Cotham, Victoria C et al. (2017) Mapping the Phosphorylation Pattern of Drosophila melanogaster RNA Polymerase II Carboxyl-Terminal Domain Using Ultraviolet Photodissociation Mass Spectrometry. ACS Chem Biol 12:153-162
Gibbs, Eric B; Lu, Feiyue; Portz, Bede et al. (2017) Phosphorylation induces sequence-specific conformational switches in the RNA polymerase II C-terminal domain. Nat Commun 8:15233
Baumann, Douglas G; Gilmour, David S (2017) A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes. Nucleic Acids Res 45:10481-10491
Li, Jian; Gilmour, David S (2015) Reconstitution of factor-dependent, promoter proximal pausing in Drosophila nuclear extracts. Methods Mol Biol 1276:133-52
Achary, Bhavana G; Campbell, Katie M; Co, Ivy S et al. (2014) RNAi screen in Drosophila larvae identifies histone deacetylase 3 as a positive regulator of the hsp70 heat shock gene expression during heat shock. Biochim Biophys Acta 1839:355-63
Li, Jian; Liu, Yingyun; Rhee, Ho Sung et al. (2013) Kinetic competition between elongation rate and binding of NELF controls promoter-proximal pausing. Mol Cell 50:711-22
Li, Jian; Gilmour, David S (2013) Distinct mechanisms of transcriptional pausing orchestrated by GAGA factor and M1BP, a novel transcription factor. EMBO J 32:1829-41
Lagha, Mounia; Bothma, Jacques P; Esposito, Emilia et al. (2013) Paused Pol II coordinates tissue morphogenesis in the Drosophila embryo. Cell 153:976-87

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