Abstract

Messenger RNA (mRNA) degradation is a process that plays an important role in the regulation of gene expression. mRNA decay rates vary greatly and can be modulated in response to environmental signals. Many studies have demonstrated that mRNA turnover can be linked to translation. One pathway that has been extensively investigated and clearly exemplifies the linked to translation. One pathway that has been extensively investigated and clearly exemplifies the link between translation and mRNA turnover is the nonsense-mediated mRNA decay pathway. The major aims of this grant are as follows. First, to rigorously define the sequences required for stimulation of decay following a nonsense codon. Second, to perform several genetic screens to identify trans acting components that function in nonsense mediated decay. Third, to utilize a series of genetic and biochemical assays to gather information about the structure and function of those trans-acting factors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM048631-06
Application #
2396061
Study Section
Molecular Biology Study Section (MBY)
Project Start
1993-01-01
Project End
2001-12-31
Budget Start
1998-01-01
Budget End
1998-12-31
Support Year
6
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Medicine & Dentistry of NJ
Department
Genetics
Type
Schools of Medicine
DUNS #
622146454
City
Piscataway
State
NJ
Country
United States
Zip Code
08854

  Publications

Wang, Weirong; Cajigas, Ivan J; Peltz, Stuart W et al. (2006) Role for Upf2p phosphorylation in Saccharomyces cerevisiae nonsense-mediated mRNA decay. Mol Cell Biol 26:3390-400
Le Roy, Florence; Salehzada, Tamim; Bisbal, Catherine et al. (2005) A newly discovered function for RNase L in regulating translation termination. Nat Struct Mol Biol 12:505-12
Gonzalez, C I; Bhattacharya, A; Wang, W et al. (2001) Nonsense-mediated mRNA decay in Saccharomyces cerevisiae. Gene 274:15-25
Wang, W; Czaplinski, K; Rao, Y et al. (2001) The role of Upf proteins in modulating the translation read-through of nonsense-containing transcripts. EMBO J 20:880-90
Gonzalez, C I; Wang, W; Peltz, S W (2001) Nonsense-mediated mRNA decay in Saccharomyces cerevisiae: a quality control mechanism that degrades transcripts harboring premature termination codons. Cold Spring Harb Symp Quant Biol 66:321-8
Wilusz, C J; Wang, W; Peltz, S W (2001) Curbing the nonsense: the activation and regulation of mRNA surveillance. Genes Dev 15:2781-5
Gonzalez, C I; Ruiz-Echevarria, M J; Vasudevan, S et al. (2000) The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay. Mol Cell 5:489-99
Ruiz-Echevarria, M J; Peltz, S W (2000) The RNA binding protein Pub1 modulates the stability of transcripts containing upstream open reading frames. Cell 101:741-51
Bhattacharya, A; Czaplinski, K; Trifillis, P et al. (2000) Characterization of the biochemical properties of the human Upf1 gene product that is involved in nonsense-mediated mRNA decay. RNA 6:1226-35
Czaplinski, K; Majlesi, N; Banerjee, T et al. (2000) Mtt1 is a Upf1-like helicase that interacts with the translation termination factors and whose overexpression can modulate termination efficiency. RNA 6:730-43

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