We will develop the methods and tools that will profile glycans in human fluids to enable the discovery of markers for diseases. Cancer is a disease known to accompany aberrant glycosylation. Glycoproteins shed by diseased cells will be harvested for their glycan content and profiled to obtain disease markers. This approach represents a new paradigm for disease marker discovery. It will focus primarily on the glycans as disease markers while neglecting, at first, the identity of the associated proteins. This approach will be multifaceted and primarily glycocentric;it contrasts and complements the proteome-centered approaches currently under extensive investigations. In this process, we will develop a set of mass spectrometry-based tools for clinical glycomics. These tools will be used to observe changes in glycosylation of diseases in two human fluids, sera and tear. The fluids are chosen by the needs of our collaborator in diseases that include ovarian cancer and ocular rosacea but will have direct implications in breast cancer and prostate cancer for sera and other dry eye diseases for tear fluids. While disease markers will be the future outcome of these studies, they will not be the only focus of this project. Instead we will also develop the tools that will be used by our collaborators to discover markers for these diseases and lay the foundation for the use of mass spectrometry in clinics for glycomic analyses.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Project (R01)
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Special Emphasis Panel (ZRG1-BCMB-M (10))
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Edmonds, Charles G
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University of California Davis
Schools of Arts and Sciences
United States
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Park, Dayoung; Arabyan, Narine; Williams, Cynthia C et al. (2016) Salmonella Typhimurium Enzymatically Landscapes the Host Intestinal Epithelial Cell (IEC) Surface Glycome to Increase Invasion. Mol Cell Proteomics 15:3653-3664
Yang, Nan; Goonatilleke, Elisha; Park, Dayoung et al. (2016) Quantitation of Site-Specific Glycosylation in Manufactured Recombinant Monoclonal Antibody Drugs. Anal Chem 88:7091-100
Arabyan, Narine; Park, Dayoung; Foutouhi, Soraya et al. (2016) Salmonella Degrades the Host Glycocalyx Leading to Altered Infection and Glycan Remodeling. Sci Rep 6:29525
Huang, Jincui; Guerrero, Andres; Parker, Evan et al. (2015) Site-specific glycosylation of secretory immunoglobulin A from human colostrum. J Proteome Res 14:1335-49
Ruhaak, L Renee; Lebrilla, Carlito B (2015) Applications of Multiple Reaction Monitoring to Clinical Glycomics. Chromatographia 78:335-342
Song, Ting; Aldredge, Danielle; Lebrilla, Carlito B (2015) A Method for In-Depth Structural Annotation of Human Serum Glycans That Yields Biological Variations. Anal Chem 87:7754-62
Dallas, David C; Guerrero, Andres; Parker, Evan A et al. (2015) Current peptidomics: applications, purification, identification, quantification, and functional analysis. Proteomics 15:1026-38
Park, Dayoung; Brune, Kristin A; Mitra, Anupam et al. (2015) Characteristic Changes in Cell Surface Glycosylation Accompany Intestinal Epithelial Cell (IEC) Differentiation: High Mannose Structures Dominate the Cell Surface Glycome of Undifferentiated Enterocytes. Mol Cell Proteomics 14:2910-21
Hong, Qiuting; Ruhaak, L Renee; Stroble, Carol et al. (2015) A Method for Comprehensive Glycosite-Mapping and Direct Quantitation of Serum Glycoproteins. J Proteome Res 14:5179-92
Ruhaak, L Renee; Taylor, Sandra L; Stroble, Carol et al. (2015) Differential N-Glycosylation Patterns in Lung Adenocarcinoma Tissue. J Proteome Res 14:4538-49

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