The investigator's goal is to understand the mechanism and regulation of flagellar motility. They have taken advantage of the powerful genetic tools available in Chlamydomonas, coupled with newly developed techniques to tag and clone genes by insertional mutagenesis, to begin a molecular dissection of function of the central pair complex of the flagellar axoneme.
The specific aims are: 1) to clone and characterize additional genes for central apparatus components; 2) to identify proteins interacting with components of the central apparatus; 3) to define functional domains required for assembly and function of individual central apparatus components using in vitro mutagenesis. The amramdillo repeat protein PF16, will be the first target for detailed molecular analysis. The investigator states that the study of central apparatus components will not only have an impact on the field of flagellar motility but also on the fields of motor regulation and microtubule function.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM051379-06
Application #
6019015
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1994-08-01
Project End
2002-08-31
Budget Start
1999-09-01
Budget End
2000-08-31
Support Year
6
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Genetics
Type
Schools of Arts and Sciences
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455