The purpose of this work is to characterize the genetic changes that occur during speciation. One of our goals is to analyze the genetics of postzygotic isolation in Drosophila;we will focus on the role of meiotic drive in the evolution of postzygotic isolation. We also propose to turn to the study of prezygotic (behavioral) isolation in Drosophila;our goal is to identify and characterize the actual genes that cause behavioral isolation between species. Using genetic and molecular population genetic approaches, we will address the following questions: 1) Does meiotic drive cause the evolution of hybrid sterility? 2) What is the identity of the genes that cause hybrid sterility and hybrid meiotic drive? 3) What is the identity of the genes that cause behavioral isolation between species? 4) Do these genes, like the ones that cause postzygotic isolation, evolve rapidly between species? 5) If so, is this rapid evolution driven by positive natural selection? We will attempt to answer these questions by analysis of D. pseudoobscura Bogota-USA and D. sechellia-D. mauritiana hybrids. Our long-term goal is to understand how animal species, including humans, arise by determining the genetic causes of speciation.

Public Health Relevance

Our goal is to understand how animal species, including humans, arise by discovering the genetic causes of speciation. This goal is relevant to NIH's mission as recent studies have shown that analysis of species hybrids provides a surprising route to discovery of functionally important genes that- despite their significance- change rapidly between populations or species.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM051932-17
Application #
8245201
Study Section
Genetic Variation and Evolution Study Section (GVE)
Program Officer
Eckstrand, Irene A
Project Start
1995-05-01
Project End
2014-03-31
Budget Start
2012-04-01
Budget End
2014-03-31
Support Year
17
Fiscal Year
2012
Total Cost
$289,602
Indirect Cost
$102,157
Name
University of Rochester
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
041294109
City
Rochester
State
NY
Country
United States
Zip Code
14627
Phadnis, Nitin (2011) Genetic architecture of male sterility and segregation distortion in Drosophila pseudoobscura Bogota-USA hybrids. Genetics 189:1001-9
Orr, H Allen (2009) Fitness and its role in evolutionary genetics. Nat Rev Genet 10:531-9
Unckless, R L; Orr, H A (2009) Dobzhansky-Muller incompatibilities and adaptation to a shared environment. Heredity 102:214-7
Phadnis, Nitin; Orr, H Allen (2009) A single gene causes both male sterility and segregation distortion in Drosophila hybrids. Science 323:376-9
Innan, Hideki; Kim, Yuseob (2004) Pattern of polymorphism after strong artificial selection in a domestication event. Proc Natl Acad Sci U S A 101:10667-72
Orr, H Allen; Masly, John P; Presgraves, Daven C (2004) Speciation genes. Curr Opin Genet Dev 14:675-9