Ionizing radiation, mutagenic chemicals, and replication of a damaged DNA template create DNA double-stranded breaks. If not handled properly, these breaks can lead to gross chromosome rearrangements and genome destabilization. Homologous recombination represents a major, conserved mechanism for the elimination of DNA double-strand breaks. Importantly, there is compelling evidence showing that even a minor deficiency in HR leads to disorders with a strong cancer predisposition in humans. Understanding the mechanisms of DSB repair by HR is highly relevant to radiation biology and also cancer biology. Over the past several decades, there has been great progress in identifying key enzymes and dissecting the mechanisms of HR, with major contributions coming from studies in the budding yeast S. cerevisiae. In this renewal project, we will tackle several outstanding questions germane for delineating the multi-faceted roles of the conserved DNA helicases Pif1 and Mph1 in the DNA synthesis step of recombination and recombination pathway choice, respectively. Moreover, we will determine how the Smc5-Smc6 complex influences the activity of the Mph1 helicase in DNA replication fork repair. The results from our continuing efforts will advance our understanding of the formation and resolution of DNA intermediates during the homologous repair of damaged chromosomes.

Public Health Relevance

Our studies focus on the mechanism by which eukaryotic cells eliminate highly mutagenic lesions, such as DNA double-stranded breaks that are induced by ionizing radiation and chemicals, from chromosomes. Importantly, defective chromosome damage repair is the underlying cause of several cancer-prone diseases and can pre-dispose affected individuals to a variety of cancers, including breast, ovarian, and pancreatic cancers. For these reasons, the studies outlined in the renewal application have direct relevance to public health.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Project (R01)
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Study Section
Radiation Therapeutics and Biology Study Section (RTB)
Program Officer
Janes, Daniel E
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Yale University
Schools of Medicine
New Haven
United States
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Zhao, Qi; Saro, Dorina; Sachpatzidis, Aristidis et al. (2014) The MHF complex senses branched DNA by binding a pair of crossover DNA duplexes. Nat Commun 5:2987
Daley, James M; Chiba, Tamara; Xue, Xiaoyu et al. (2014) Multifaceted role of the Topo III?-RMI1-RMI2 complex and DNA2 in the BLM-dependent pathway of DNA break end resection. Nucleic Acids Res 42:11083-91
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Anand, Ranjith P; Shah, Kartik A; Niu, Hengyao et al. (2012) Overcoming natural replication barriers: differential helicase requirements. Nucleic Acids Res 40:1091-105
Busygina, Valeria; Saro, Dorina; Williams, Gareth et al. (2012) Novel attributes of Hed1 affect dynamics and activity of the Rad51 presynaptic filament during meiotic recombination. J Biol Chem 287:1566-75

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