The function of the kidneys is to maintain normal ionic concentrations in the blood. Normally the kidneys maintain the blood ionic composition within very tight range and imbalance produces very serious health consequences including death. The goal of this grant is to understand the ion channels that function in the kidney to transport chloride and maintain chloride homeostasis in the body. This application will focus on an exciting new family of chloride channels that was just discovered, the anoctamins (also called TMEM16). We have preliminary data that several anoctamins are expressed in kidney and are likely to play important roles in chloride secretion and/or absorption. This application will investigate the expression of anotamins in normal mouse and human kidney and in kidney from human and from mouse models of polycystic kidney disease. Expression will be determined using quantitative RT-PCR, western blot, and immunofluorescent confocal microscopy. The functional structure and organization of the anoctamin channel will be investigated using electrophysiological (whole-cell and single channel patch clamp recording) and biochemical analysis of cells expressing Ano1. The location of Ca2+ binding sites and mechanisms of regulation of Ano1 by voltage and Ca2+ will be explored to develop a quantitative model of how anoctamin channels are gated.

Public Health Relevance

Kidneys function to filter approximately 200 liters of blood per day in order to remove toxic substances and to regulate the ionic composition of the body. These studies will elucidate the mechanisms of chloride transport by the kidney and will make inroads into identifying mechanisms that hopefully will be useful in treating various kinds of kidney disease including diabetic kidney disease, high blood pressure, autoimmune diseases, and genetic diseases such as polycystic kidney disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM060448-10
Application #
8248810
Study Section
Cellular and Molecular Biology of the Kidney Study Section (CMBK)
Program Officer
Deatherage, James F
Project Start
2000-07-01
Project End
2015-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
10
Fiscal Year
2012
Total Cost
$344,100
Indirect Cost
$122,100
Name
Emory University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
066469933
City
Atlanta
State
GA
Country
United States
Zip Code
30322
Yu, Kuai; Zhu, Jinqiu; Qu, Zhiqiang et al. (2014) Activation of the Ano1 (TMEM16A) chloride channel by calcium is not mediated by calmodulin. J Gen Physiol 143:253-67
Xiao, Qinghuan; Cui, Yuanyuan (2014) Acidic amino acids in the first intracellular loop contribute to voltage- and calcium- dependent gating of anoctamin1/TMEM16A. PLoS One 9:e99376
Rui, Yanfang; Myers, Kenneth R; Yu, Kuai et al. (2013) Activity-dependent regulation of dendritic growth and maintenance by glycogen synthase kinase 3*. Nat Commun 4:2628
Hartzell, H Criss; Ruppersburg, Chelsey Chandler (2013) Functional reconstitution of a chloride channel bares its soul. Proc Natl Acad Sci U S A 110:19185-6
Duran, Charity; Chien, Li-Ting; Hartzell, H Criss (2013) Drosophila bestrophin-1 currents are regulated by phosphorylation via a CaMKII dependent mechanism. PLoS One 8:e58875
Oh, Soo-Jin; Hwang, Seok Jin; Jung, Jonghoon et al. (2013) MONNA, a potent and selective blocker for transmembrane protein with unknown function 16/anoctamin-1. Mol Pharmacol 84:726-35
Duran, Charity; Qu, Zhiqiang; Osunkoya, Adeboye O et al. (2012) ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins. Am J Physiol Cell Physiol 302:C482-93
Yu, Kuai; Duran, Charity; Qu, Zhiqiang et al. (2012) Explaining calcium-dependent gating of anoctamin-1 chloride channels requires a revised topology. Circ Res 110:990-9
Xiao, Qinghuan; Yu, Kuai; Perez-Cornejo, Patricia et al. (2011) Voltage- and calcium-dependent gating of TMEM16A/Ano1 chloride channels are physically coupled by the first intracellular loop. Proc Natl Acad Sci U S A 108:8891-6
Sheridan, John T; Worthington, Erin N; Yu, Kuai et al. (2011) Characterization of the oligomeric structure of the Ca(2+)-activated Cl- channel Ano1/TMEM16A. J Biol Chem 286:1381-8

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