In sum, overall the project suffered an estimated 3 month set-back. We have requested supplementary funding for 3 months (25% of the current years direct cost award of $192,456 or $48,144) to cover this set-back. The primary request is to recoup salary associated with lost time (postdocs and PI salary) and a small amount to offset the cost of reagents and lab supplies required to recover experiments that were on-going at the time of the storm and to replace reagents for future use. Please see budget justification for additional details.
Germ cells are the link from generation to generation and must proliferate to ensure fertility; and germ cell proliferation is controlled by interaction with neighboring cells and by hormones and nutrition. We study conserved molecular pathways that control germ cell proliferation. These investigations inform similar processes in humans with implications for fertility; cancer; and stem cell biology.
|Pekar, Olga; Ow, Maria C; Hui, Kailyn Y et al. (2017) Linking the environment, DAF-7/TGF? signaling and LAG-2/DSL ligand expression in the germline stem cell niche. Development 144:2896-2906|
|Roy, Debasmita; Michaelson, David; Hochman, Tsivia et al. (2016) Cell cycle features of C. elegans germline stem/progenitor cells vary temporally and spatially. Dev Biol 409:261-71|
|Atwell, Kathryn; Dunn, Sara-Jane; Osborne, James M et al. (2016) How computational models contribute to our understanding of the germ line. Mol Reprod Dev 83:944-957|
|Deng, Xinzhu; Michaelson, David; Tchieu, Jason et al. (2015) Targeting Homologous Recombination in Notch-Driven C. elegans Stem Cell and Human Tumors. PLoS One 10:e0127862|
|Atwell, Kathryn; Qin, Zhao; Gavaghan, David et al. (2015) Mechano-logical model of C. elegans germ line suggests feedback on the cell cycle. Development 142:3902-11|
|Qin, Zhao; Hubbard, E Jane Albert (2015) Non-autonomous DAF-16/FOXO activity antagonizes age-related loss of C. elegans germline stem/progenitor cells. Nat Commun 6:7107|
|Hubbard, E Jane Albert (2014) FLP/FRT and Cre/lox recombination technology in C. elegans. Methods 68:417-24|
|Hubbard, E Jane Albert; Korta, Dorota Z; Dalfó, Diana (2013) Physiological control of germline development. Adv Exp Med Biol 757:101-31|
|Korta, Dorota Z; Tuck, Simon; Hubbard, E Jane Albert (2012) S6K links cell fate, cell cycle and nutrient response in C. elegans germline stem/progenitor cells. Development 139:859-70|
|Dalfó, Diana; Michaelson, David; Hubbard, E Jane Albert (2012) Sensory regulation of the C. elegans germline through TGF-?-dependent signaling in the niche. Curr Biol 22:712-9|
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