Members of transforming growth factor-beta (TFG-beta) superfamily are multifunctional growth factors that control a broad range of cellular responses in metazoan organism. TGF- betas inhibit the growth of most epithelial and hematopoietic cells and regulates the production of extracellular matrix by mesenchymal cells. Loss of the antiproliferative responsiveness to TGF-beta is often considered as a major step in tumor progression. The long-term objective of this application is to understand the molecular basis of how alterations in TGF-beta antiproliferative signaling pathways lead to deregulation of growth control in human diseases. The general strategy of this application is to focus on the mechanism of ubiquitin/proteasome- dependent degradation of tumor suppressor Smad2. Smad2 is an important TGF-beta signal transducer that inhibits cell proliferation though transcription-dependent mechanisms. However, the precise regulation of Smad2 remains enigmatic. The unifying hypothesis of this proposal is that ubiquitin/proteasome pathway targets Smad2 for degradation through a specific ubiquitin E3 ligase. To test this hypothesis, we will study the mechanism of Smad2 degradation by a newly identified ubiquitin E3 ligase called Smurf2 and investigate the physiological consequences of deregulation of Smurf2 activity in Smad2 degradation.
Three Specific Aims are proposed: 1. Define the molecular mechanism of Smurf2-mediated Smad2 degradation. The physical interaction between Smurf2and Smad2, the structural domains required for the interaction, and the molecular basis for substrate selectivity will be determined. 2. Investigate the physiological impact of Smurf2-mediated Smad2 degradation in TGF- beta signaling. It will be tested how Smurf2-mediated Smad2 degradation affects Smad2 signaling during TGF-beta induced cell cycle arrest and Xenopus early embryonic development. 3. Examine the regulation of Smurf2-Smad2 degradation in epithelial cells. Molecular and biochemical approaches will be used to define the cellular mechanism of Smurf2-mediated Smad2 degradation and to analyze differential Smad2 degradation in normal and cancer cells. The proposed studies should help to establish a working theory for ubiquitination-mediated Smad2 turnover during TGF- beta-induced growth inhibition and to understand the mechanisms of Smad actions in malignant transformation and progression of human cancers. Finally, the result may provide a foundation of the rational design of novel therapeutic approaches for cancer prevention and treatment.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
3R01GM063773-04S1
Application #
6920599
Study Section
Biochemical Endocrinology Study Section (BCE)
Program Officer
Zatz, Marion M
Project Start
2001-08-01
Project End
2006-07-31
Budget Start
2004-08-01
Budget End
2005-07-31
Support Year
4
Fiscal Year
2004
Total Cost
$100,976
Indirect Cost
Name
Baylor College of Medicine
Department
Surgery
Type
Schools of Medicine
DUNS #
051113330
City
Houston
State
TX
Country
United States
Zip Code
77030
Liu, Ting; Zhao, Meiling; Liu, Jinquan et al. (2017) Tumor suppressor bromodomain-containing protein 7 cooperates with Smads to promote transforming growth factor-? responses. Oncogene 36:362-372
Sun, Shuangwu; Liu, Sisi; Zhang, Zhengmao et al. (2017) Phosphatase UBLCP1 controls proteasome assembly. Open Biol 7:
Gu, Shuchen; Liu, Yanjing; Zhu, Bowen et al. (2016) Loss of ?-Tubulin Acetylation Is Associated with TGF-?-induced Epithelial-Mesenchymal Transition. J Biol Chem 291:5396-405
Liu, Sisi; Long, Jianyin; Yuan, Bo et al. (2016) SUMO Modification Reverses Inhibitory Effects of Smad Nuclear Interacting Protein-1 in TGF-? Responses. J Biol Chem 291:24418-24430
Wang, G; Yu, Y; Sun, C et al. (2016) STAT3 selectively interacts with Smad3 to antagonize TGF-?. Oncogene 35:4388-98
Sun, Chuang; Wang, Gaohang; Wrighton, Katharine H et al. (2016) Regulation of p27(Kip1) phosphorylation and G1 cell cycle progression by protein phosphatase PPM1G. Am J Cancer Res 6:2207-2220
Chen, Fenfang; Lin, Xia; Xu, Pinglong et al. (2015) Nuclear Export of Smads by RanBP3L Regulates Bone Morphogenetic Protein Signaling and Mesenchymal Stem Cell Differentiation. Mol Cell Biol 35:1700-11
Chen, Wanze; Wu, Jianfeng; Li, Lisheng et al. (2015) Ppm1b negatively regulates necroptosis through dephosphorylating Rip3. Nat Cell Biol 17:434-44
Zhao, Yulan; Xiao, Mu; Sun, Baoguo et al. (2014) C-terminal domain (CTD) small phosphatase-like 2 modulates the canonical bone morphogenetic protein (BMP) signaling and mesenchymal differentiation via Smad dephosphorylation. J Biol Chem 289:26441-50
Shen, Tao; Sun, Chuang; Zhang, Zhengmao et al. (2014) Specific control of BMP signaling and mesenchymal differentiation by cytoplasmic phosphatase PPM1H. Cell Res 24:727-41

Showing the most recent 10 out of 39 publications