? The unique features of fluorescence fluctuation spectroscopy (FFS) make this technique attractive for cellular applications. FFS determines kinetic and molecular properties of proteins with submicron resolution and single molecule sensitivity. Especially, the application of FFS to proteins tagged with a variant of green fluorescent protein has the potential to revolutionize in vivo studies. We will focus here on dual-color photon counting histogram (PCH) analysis. PCH is a relatively new FFS technique with the potential to provide quantitative information about protein association in living cells. Dual-color PCH exploits two fluorescent protein labels with distinctively different emission spectra. Separating the two colors into different detection channels strongly increases the sensitivity of PCH in detecting protein association. We will adept dual-color PCH for in vivo studies, implement global analysis methods and thoroughly characterize the technique. The long-term objective of the proposed research lies in the concurrent development and application of fluorescence fluctuation techniques, so that their full potential for in vivo studies is realized. The impact of this new technology will be felt in many biological areas with applications ranging from basic research in cell biology to pharmaceutical drug screening. Dual-color PCH will be applied to study the interactions between nuclear receptors and their coregulators in vivo. Nuclear receptors are transcription factors that turn gene expression on and off. They inhibit or enhance transcription by recruiting an array of coactivat6r or corepressor proteins. The quantitative characterization of the oligomerization state of the receptorcoregulator complex by dual-color PCH will be at the focus of this study. Nuclear receptors affect processes as diverse as reproduction, development, and general metabolism, and have been implicated in cancer, diabetes, and many other diseases. Dual-color PCH could help in fighting these diseases by providing detailed information about the protein interactions that govern gene expression in cells. ? ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM064589-04
Application #
6870930
Study Section
Special Emphasis Panel (ZRG1-ISD (01))
Program Officer
Lewis, Catherine D
Project Start
2002-02-01
Project End
2008-01-31
Budget Start
2005-02-01
Budget End
2006-01-31
Support Year
4
Fiscal Year
2005
Total Cost
$171,929
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Physics
Type
Schools of Arts and Sciences
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Li, Jinhui; Barylko, Barbara; Eichorst, John P et al. (2016) Association of Endophilin B1 with Cytoplasmic Vesicles. Biophys J 111:565-576
Hur, Kwang-Ho; Chen, Yan; Mueller, Joachim D (2016) Characterization of Ternary Protein Systems In Vivo with Tricolor Heterospecies Partition Analysis. Biophys J 110:1158-67
Smith, Elizabeth M; Hennen, Jared; Chen, Yan et al. (2015) Z-scan fluorescence profile deconvolution of cytosolic and membrane-associated protein populations. Anal Biochem 480:11-20
Smith, Elizabeth M; Hennen, Jared; Chen, Yan et al. (2015) In situ quantification of protein binding to the plasma membrane. Biophys J 108:2648-57
Hur, Kwang-Ho; Mueller, Joachim D (2015) Quantitative Brightness Analysis of Fluorescence Intensity Fluctuations in E. Coli. PLoS One 10:e0130063
Smith, Elizabeth M; Macdonald, Patrick J; Chen, Yan et al. (2014) Quantifying protein-protein interactions of peripheral membrane proteins by fluorescence brightness analysis. Biophys J 107:66-75
James, Nicholas G; Digman, Michelle A; Ross, Justin A et al. (2014) A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells. Biochim Biophys Acta 1840:315-21
Hur, Kwang-Ho; Macdonald, Patrick J; Berk, Serkan et al. (2014) Quantitative measurement of brightness from living cells in the presence of photodepletion. PLoS One 9:e97440
Fogarty, Keir H; Berk, Serkan; Grigsby, Iwen F et al. (2014) Interrelationship between cytoplasmic retroviral Gag concentration and Gag-membrane association. J Mol Biol 426:1611-24
Macdonald, Patrick J; Johnson, Jolene; Chen, Yan et al. (2014) Brightness experiments. Methods Mol Biol 1076:699-718

Showing the most recent 10 out of 41 publications