The temporal regulation of replication origin initiation timing is a form of epigenetic regulation whose significance and mechanism remain poorly understood. The origin timing program is thought to be connected with the gene expression program during development, perhaps to ensure the stable inheritance of transcriptional states. Differential origin timing is also propose to play a role in genome stability. Chromatin structure has been implicated in modulating replication timing but a precise mechanism remains vague. We have now determined that Forkhead transcription factors, Fkh1 and Fkh2 (Fkh1/2), play a major role in establishing genomic replication patterns by determining the activation of many of the most efficient, early-firing origins in the yeast genome. No other factor has been identified as having such a significant impact, in level and breadth, on genomic replication patterns. The role of Fkh1/2 in origin regulation is independent of their roles in transcriptional regulation. Instead, our results show that Fkh1/2 are required for early origin clustering or subnuclear localization and their association with the initiation factor Cdc45, both in G1-phase. These results suggest the overall hypothesis that Forkhead proteins play a key role in origin regulation by tethering origins into clusters that will become the first replication factories in S-phase. We propose that origin clustering is mediated by a novel mechanism of Fkh1/2 dimerization and/or by interactions with ORC, which we recently demonstrated. These findings have opened exciting new avenues toward understanding genome organization and mechanisms of epigenetic regulation. This proposal will examine new mechanisms of function for Forkhead transcription factors in replication that will inform our understanding of their roles in transcription regulation, and our newly hypothesized role in higher-order genome organization.
Our Specific Aims to determine the mechanisms that Fkh1 and Fkh2 use to regulate chromosome replication and structure are: 1) Develop molecular tools to analyze and manipulate origin clustering dynamics 2) Elucidate the role of Fkh1/2 binding in the mechanism of origin regulation 3) Perform structure-function analysis of Fkh1 and Fkh2 4) Characterize the long-range chromatin interactions mediated by Fkh1 and Fkh2

Public Health Relevance

The establishment and maintenance of stable epigenetic states is fundamental to normal cellular differentiation and homeostasis. This proposal investigates a novel mechanism of epigenetic regulation of DNA replication origins by Forkhead (or Fox) transcription factors involving the spatial clustering of replication origins. These studies have implications for understanding the mechanisms that cause developmental defects and cancers.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM065494-10
Application #
8666508
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Reddy, Michael K
Project Start
2003-02-01
Project End
2018-01-31
Budget Start
2014-04-15
Budget End
2015-01-31
Support Year
10
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Southern California
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
Peace, Jared M; Villwock, Sandra K; Zeytounian, John L et al. (2016) Quantitative BrdU immunoprecipitation method demonstrates that Fkh1 and Fkh2 are rate-limiting activators of replication origins that reprogram replication timing in G1 phase. Genome Res 26:365-75
Ostrow, A Zachary; Viggiani, Christopher J; Aparicio, Jennifer G et al. (2015) ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin. Methods Mol Biol 1300:155-68
Massilamany, Chandirasegaran; Marciano-Cabral, Francine; Rocha-Azevedo, Bruno da et al. (2014) SJL mice infected with Acanthamoeba castellanii develop central nervous system autoimmunity through the generation of cross-reactive T cells for myelin antigens. PLoS One 9:e98506
Ostrow, A Zachary; Nellimoottil, Tittu; Knott, Simon R V et al. (2014) Fkh1 and Fkh2 bind multiple chromosomal elements in the S. cerevisiae genome with distinct specificities and cell cycle dynamics. PLoS One 9:e87647
Peace, Jared M; Ter-Zakarian, Anna; Aparicio, Oscar M (2014) Rif1 regulates initiation timing of late replication origins throughout the S. cerevisiae genome. PLoS One 9:e98501
Villwock, Sandra K; Aparicio, Oscar M (2014) Two-dimensional agarose gel electrophoresis for analysis of DNA replication. Methods Mol Biol 1205:329-40
Aparicio, Oscar M (2013) Location, location, location: it's all in the timing for replication origins. Genes Dev 27:117-28
Pope, Benjamin D; Aparicio, Oscar M; Gilbert, David M (2013) SnapShot: Replication timing. Cell 152:1390-1390.e1
Zhong, Yuan; Nellimoottil, Tittu; Peace, Jared M et al. (2013) The level of origin firing inversely affects the rate of replication fork progression. J Cell Biol 201:373-83
Knott, Simon R V; Peace, Jared M; Ostrow, A Zachary et al. (2012) Forkhead transcription factors establish origin timing and long-range clustering in S. cerevisiae. Cell 148:99-111

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