The folding of proteins to their native structures is key to their function and malfunction in the cells of living organisms. Protein folding occurs on a high dimensionality and complex surface, whose features are often hidden in standard ensemble studies of protein folding. In this project, we will continue to improve and develop novel single molecule fluorescence methodologies to probe such complex features of protein folding. We will focus on understanding the folding properties of two amyloidogenic proteins, Sup 35 and ?-synuclein. The misfolding and aggregation of such amyloidogenic proteins are implicated in a host of diseases including Mad Cow and Parkinson's (?-synuclein). Additionally, mounting evidence recently implicates the aggregation of these proteins in biologically constructive roles, such as acting as a protein-only genetic vehicle in yeast (Sup 35). Hence, a detailed understanding of the folding and dynamics of such proteins is very important from the point of view of human health. We will build on our strong foundation of single molecule investigations of biological folding to further develop and apply a suite of single molecule fluorescence methods, including single molecule FRET, polarization and correlation spectroscopy, in combination with novel and powerful microfluidic methods and protein engineering to gain insights into the folding of these proteins, both as monomeric species, as well as during the early stages of the aggregation process. Our studies will uncover whether these monomeric proteins (both understood to be intrinsically disordered) have elements of residual structure, how repeat sequences influence their folding and dynamics, and how other key cellular factors such as chaperones (for Sup 35) and binding to membranes (for ?-synuclein) influence the folding and structural dynamics of such proteins. Furthermore, by monitoring their folding properties during the early stages of aggregation, we aim to understand how these two processes are coupled within the context of this highly complex and heterogenous mixture of oligomeric species, insight that will be valuable in the understanding of the molecular and structural mechanisms of protein amyloidosis. Finally, these insights are anticipated to be extremely valuable in the design of therapeutic strategies to combat amyloid diseases.

Public Health Relevance

This project aims to develop and apply novel single molecule fluorescence methodologies to probe complex folding features of amyloidogenic proteins. Mechanistic insights gained in these studies will be key in understanding the structural biology of amyloidogenic proteins and protein aggregation, which are implicated in diseases such as Parkinson's and Prion diseases. Insights obtained are expected to be valuable during the design of therapeutic strategies to prevent or reverse such diseases, thus contributing to improving public health.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM066833-09
Application #
8232074
Study Section
Macromolecular Structure and Function C Study Section (MSFC)
Program Officer
Lewis, Catherine D
Project Start
2003-06-01
Project End
2014-02-28
Budget Start
2012-03-01
Budget End
2014-02-28
Support Year
9
Fiscal Year
2012
Total Cost
$574,015
Indirect Cost
$271,743
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Lee, Taehyung; Moran-Gutierrez, Crystal R; Deniz, Ashok A (2015) Probing protein disorder and complexity at single-molecule resolution. Semin Cell Dev Biol 37:26-34
Moosa, Mahdi Muhammad; Ferreon, Allan Chris M; Deniz, Ashok A (2015) Forced folding of a disordered protein accesses an alternative folding landscape. Chemphyschem 16:90-4
Banerjee, Priya R; Deniz, Ashok A (2014) Shedding light on protein folding landscapes by single-molecule fluorescence. Chem Soc Rev 43:1172-88
Ferreon, Allan Chris M; Ferreon, Josephine C; Wright, Peter E et al. (2013) Modulation of allostery by protein intrinsic disorder. Nature 498:390-4
Ferreon, Allan Chris M; Deniz, Ashok A (2011) Protein folding at single-molecule resolution. Biochim Biophys Acta 1814:1021-9
Tyagi, Navneet K; Fenton, Wayne A; Deniz, Ashok A et al. (2011) Double mutant MBP refolds at same rate in free solution as inside the GroEL/GroES chaperonin chamber when aggregation in free solution is prevented. FEBS Lett 585:1969-72
Gambin, Yann; VanDelinder, Virginia; Ferreon, Allan Chris M et al. (2011) Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing. Nat Methods 8:239-41
Berezhna, Svitlana Yu; Supekova, Lubica; Sever, Mary J et al. (2011) Dual regulation of hepatitis C viral RNA by cellular RNAi requires partitioning of Ago2 to lipid droplets and P-bodies. RNA 17:1831-45
Gambin, Yann; Deniz, Ashok A (2010) Multicolor single-molecule FRET to explore protein folding and binding. Mol Biosyst 6:1540-7
Ferreon, Allan Chris M; Moran, Crystal R; Ferreon, Josephine C et al. (2010) Alteration of the alpha-synuclein folding landscape by a mutation related to Parkinson's disease. Angew Chem Int Ed Engl 49:3469-72

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