Nitrogenase reaction represents a major source of the usable form of nitrogen that supports the existence of human population. As such, understanding how small building blocks are assembled into a functional nitrogenase entity is of significant relevance to human health. Using combined genetic, biochemical, spectroscopic and structural approaches, we propose to investigate how M-cluster, the active center of molybdenum nitrogenase, is assembled. Specifically, we will examine the formation of a Fe-S core of M-cluster, the maturation of Fe-S core into an M-cluster, and the transfer of M-cluster from the assembly site to its target location in great detail. Through our proposed studies, we expect to refine the biosynthetic pathway of M-cluster, which will provide crucial insights into th structural-functional relationship of nitrogenase and the general assembly mechanism of complex metal clusters in biological systems.

Public Health Relevance

Using combined genetic, biochemical, spectroscopic and structural approaches, we propose to investigate how M-cluster, the active center of molybdenum nitrogenase, is assembled. Through our proposed studies, we expect to refine the biosynthetic mechanism of M-cluster, which will provide crucial insights into the structural-functional relationship of nitrogenase and the general assembly scheme of complex metal clusters in biological systems.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM067626-10
Application #
8371547
Study Section
Macromolecular Structure and Function A Study Section (MSFA)
Program Officer
Anderson, Vernon
Project Start
2003-05-01
Project End
2016-11-30
Budget Start
2012-12-15
Budget End
2013-11-30
Support Year
10
Fiscal Year
2013
Total Cost
$308,127
Indirect Cost
$103,127
Name
University of California Irvine
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Rettberg, Lee A; Wilcoxen, Jarett; Lee, Chi Chung et al. (2018) Probing the coordination and function of Fe4S4 modules in nitrogenase assembly protein NifB. Nat Commun 9:2824
Tanifuji, Kazuki; Lee, Chi Chung; Sickerman, Nathaniel S et al. (2018) Tracing the 'ninth sulfur' of the nitrogenase cofactor via a semi-synthetic approach. Nat Chem 10:568-572
Rupnik, Kresimir; Lee, Chi Chung; Hu, Yilin et al. (2018) A VTVH MCD and EPR Spectroscopic Study of the Maturation of the ""Second"" Nitrogenase P-Cluster. Inorg Chem 57:4719-4725
Sickerman, Nathaniel S; Rettberg, Lee A; Lee, Chi Chung et al. (2017) Cluster assembly in nitrogenase. Essays Biochem 61:271-279
Hu, Yilin; Ribbe, Markus W (2016) Maturation of nitrogenase cofactor-the role of a class E radical SAM methyltransferase NifB. Curr Opin Chem Biol 31:188-94
Lee, Chi Chung; Sickerman, Nathaniel S; Hu, Yilin et al. (2016) YedY: A Mononuclear Molybdenum Enzyme with a Redox-Active Ligand? Chembiochem 17:453-5
Fay, Aaron W; Blank, Michael A; Rebelein, Johannes G et al. (2016) Assembly scaffold NifEN: A structural and functional homolog of the nitrogenase catalytic component. Proc Natl Acad Sci U S A 113:9504-8
Lee, Chi Chung; Fay, Aaron W; Weng, Tsu-Chien et al. (2015) Uncoupling binding of substrate CO from turnover by vanadium nitrogenase. Proc Natl Acad Sci U S A 112:13845-9
Wiig, Jared A; Hu, Yilin; Ribbe, Markus W (2015) Refining the pathway of carbide insertion into the nitrogenase M-cluster. Nat Commun 6:8034
Cahn, Jackson K B; Brinkmann-Chen, Sabine; Spatzal, Thomas et al. (2015) Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases. Biochem J 468:475-84

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