Messenger RNA localization plays a key role in creating the asymmetric distributions of proteins necessary for cellular and developmental polarity. The sorting of specific mRNAs to different subcellular domains is a complex process involving the assembly and trafficking of large ribonucleoprotein (RNP) complexes. How specificity is conferred on this process, particularly in cells where several mRNAs localization pathways operate concurrently, is poorly understood. This proposal integrates biochemical, genetic, and imaging-based approaches to investigate how mRNAs are specifically recognized and packaged into localization competent particles, how these RNP particles are adapted to different localization mechanisms, the extent to which localization pathways are interconnected, and how multiple localization pathways are coordinated within a single cell. The Drosophila oocyte and early embryo provide ideal model systems for these studies because they are equipped with several mechanistically distinct trafficking pathways that direct the localization of mRNAs essential for axis formation and germline development.
In Aim 1, we will investigate mechanisms of RNP particle assembly using biochemical approaches including tandem RNA affinity purification to isolate and characterize the components of Nanos RNP complexes. These studies will be complemented in Aim 2 by two imaging-based approaches that investigate whether concurrent trafficking pathways are coordinated through the sharing of RNP particles by different mRNAs.
Aim 3 expands our studies to determine the broader significance of mRNA localization in the development and function of polarized cells through a novel genome- wide screen for transcripts with asymmetric subcellular distributions in neurons. The identification of new localized mRNAs in this screen and screens in other cell types performed by our collaborators will shed light on determinants of mRNA targeting specificity and may uncover novel roles for localized mRNAs in development and function of polarized cells.

Public Health Relevance

Messenger RNA localization is an important mechanism for producing proteins in particular regions of cells where their functions are needed and plays a well documented role in animal development and in the formation and function of polarized cells like motile fibroblasts and neurons. Mutations in proteins that regulate messenger RNA localization have been associated with a variety of cancers and disruption of neuronal messenger RNA localization may contribute to mental retardation and neuromuscular disorders. The proposed studies will shed light on the mechanisms used to move mRNAs to their specific destinations and how the disruption of this process may lead to diseases like cancer or neurological dysfunction.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
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Hoodbhoy, Tanya
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Princeton University
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Xu, Xin; Brechbiel, Jillian L; Gavis, Elizabeth R (2013) Dynein-dependent transport of nanos RNA in Drosophila sensory neurons requires Rumpelstiltskin and the germ plasm organizer Oskar. J Neurosci 33:14791-800
JayaNandanan, N; Gavis, Elizabeth R; Riechmann, Veit et al. (2011) A genetic in vivo system to detect asymmetrically distributed RNA. EMBO Rep 12:1167-74
Lerit, Dorothy A; Gavis, Elizabeth R (2011) Transport of germ plasm on astral microtubules directs germ cell development in Drosophila. Curr Biol 21:439-48
Sinsimer, Kristina S; Jain, Roshan A; Chatterjee, Seema et al. (2011) A late phase of germ plasm accumulation during Drosophila oogenesis requires lost and rumpelstiltskin. Development 138:3431-40
Becalska, Agata N; Kim, YoungJung R; Belletier, Nicolette G et al. (2011) Aubergine is a component of a nanos mRNA localization complex. Dev Biol 349:46-52
Becalska, Agata N; Gavis, Elizabeth R (2010) Bazooka regulates microtubule organization and spatial restriction of germ plasm assembly in the Drosophila oocyte. Dev Biol 340:528-38
Weil, Timothy T; Xanthakis, Despina; Parton, Richard et al. (2010) Distinguishing direct from indirect roles for bicoid mRNA localization factors. Development 137:169-76
Becalska, Agata N; Gavis, Elizabeth R (2009) Lighting up mRNA localization in Drosophila oogenesis. Development 136:2493-503
Jaramillo, Angela M; Weil, Timothy T; Goodhouse, Joseph et al. (2008) The dynamics of fluorescently labeled endogenous gurken mRNA in Drosophila. J Cell Sci 121:887-94
Weil, Timothy T; Parton, Richard; Davis, Ilan et al. (2008) Changes in bicoid mRNA anchoring highlight conserved mechanisms during the oocyte-to-embryo transition. Curr Biol 18:1055-61

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