Natural products have a proven record of providing a significant fraction, either directly or as lead compounds, of human medicines. Among natural products, the terpenoids (isoprenoids) stand out as being the largest class (>50,000 already known), with the 20-carbon diterpenoids targeted here forming a significant fraction of these (>12,000 known). Indeed, the extensive diversification of diterpenoids indicates that the manifold structures that can be formed from this C20 backbone provide a rich source of biological activity, and a number of these natural products are used as pharmaceuticals (e.g., the anti- cancer paclitaxel/TaxolTM and anti-biotic mutilins) or research tools (e.g., the protein kinase C activating and tumor promoting phorbol esters, and adenylate cyclase activator forskolin). Conversely, we have recently discovered that the human pathogen Mycobacterium tuberculosis utilizes a diterpenoid, isotuberculosinol, to modulate our initial immune response to infection. Accordingly, we propose here to continue our productive investigations of the enzymes, from both plants and microbes, that catalyze formation of the underlying hydrocarbon skeletal structures. Specifically, we will build on our findings from the previous grant period, which includes not only the discovery of isotuberculosinol, but elucidation of some of the first diterpene synthase crystal structures as well. This proposal then advances our long- term goal of engineering enzymes and metabolic pathways for the production of targeted libraries and specific individual terpenoid 'natural'products for pharmaceutical investigation and use. In particular, the objective of this proposal is a detailed structure-function investigation of the synthases/cyclases that catalyze formation of the hydrocarbon skeletons underlying the structural diversity of diterpenoid natural products. These enzymes catalyze complex electrophilic reactions that form new carbon-carbon bonds, and play important roles in initiating diterpenoid biosynthesis. Accordingly, our studies have implications not only for providing increased access to such natural products, but also insights into potential drug targets, specifically in the widespread pathogen M. tuberculosis.
The diterpenoids form a very large class of natural products (>12,000 known), which includes many of realized (e.g., TaxolTM) or potential (e.g., prostratin) pharmaceutical use, as well as a compound (isotuberculosinol) that we have recently discovered is produced by the human pathogen Mycobacterium tuberculosis to suppress our immune response to infection. Here we propose to continue our fruitful investigations of the enzymes that form the hydrocarbon backbones that underlie the structural diversity of these natural products. This will not only increase our understanding of the relevant enzymatic mechanisms for engineering such biosynthesis to increase access to these often scarce natural products, but also provide insights into these enzymes as potential drugs targets against the pernicious M. tuberculosis.
|Liang, Jin; Liu, Jiang; Brown, Reid et al. (2018) Direct production of dihydroxylated sesquiterpenoids by a maize terpene synthase. Plant J 94:847-856|
|O'Brien, Terrence E; Bertolani, Steven J; Zhang, Yue et al. (2018) Predicting Productive Binding Modes for Substrates and Carbocation Intermediates in Terpene Synthases-Bornyl Diphosphate Synthase as a Representative Case. ACS Catal 8:3322-3330|
|Schulte, Samuel; Potter, Kevin C; Lemke, Cody et al. (2018) Catalytic Bases and Stereocontrol in Lamiaceae Class II Diterpene Cyclases. Biochemistry 57:3473-3479|
|Nagel, Raimund; Thomas, Jill A; Adekunle, Faith A et al. (2018) Arginine in the FARM and SARM: A Role in Chain-Length Determination for Arginine in the Aspartate-Rich Motifs of Isoprenyl Diphosphate Synthases from Mycobacterium tuberculosis. Molecules 23:|
|Xu, Meimei; Jia, Meirong; Hong, Young J et al. (2018) Premutilin Synthase: Ring Rearrangement by a Class II Diterpene Cyclase. Org Lett 20:1200-1202|
|Jia, Meirong; O'Brien, Terrence E; Zhang, Yue et al. (2018) Changing Face: A Key Residue for the Addition of Water by Sclareol Synthase. ACS Catal 8:3133-3137|
|Inabuy, Fainmarinat S; Fischedick, Justin T; Lange, Iris et al. (2017) Biosynthesis of Diterpenoids in Tripterygium Adventitious Root Cultures. Plant Physiol 175:92-103|
|Jia, Meirong; Zhou, Ke; Tufts, Samuel et al. (2017) A Pair of Residues That Interactively Affect Diterpene Synthase Product Outcome. ACS Chem Biol 12:862-867|
|Xu, Meimei; Hillwig, Matthew L; Tiernan, Mollie S et al. (2017) Probing Labdane-Related Diterpenoid Biosynthesis in the Fungal Genus Aspergillus. J Nat Prod 80:328-333|
|Jia, Meirong; Peters, Reuben J (2017) cis or trans with class II diterpene cyclases. Org Biomol Chem 15:3158-3160|
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