The """"""""chemical nose/tongue"""""""" approach presents a potential alternative to specific recognition and separations techniques. In this approach a sensor array is generated to provide differential interaction with analytes via selective receptors, generating a stimulus response pattern that can be statistically analyzed and used for the identification of individual target analytes and also analysis of complex mixtures. Recently, we have developed nanoparticle-fluorescent polymer sensors for identification of proteins, bacteria, and cancerous cells through a fluorophore-displacement mechanism as well as a highly sensitive nanoparticle-GFP based """"""""chemical nose"""""""" strategy for protein detection in biofluid. Current chemical nose sensors for proteins and cell-surfaces are single channel, meaning a separate well or channel is required for each sensing element. This requirement for spatially distinct sensor elements complicates both microplate-based techniques and the application of array-based sensing in other venues, including microfluidics platforms and tissue staining. To overcome this limitation, we will exploit the spectral range of fluorescent proteins (FPs) to provide multi-channel fluorescence transduction for sensing applications. Multi-channel sensing will facilitate implementation of array-based sensing, allowing """"""""one well"""""""" sensing while improving sensitivity through generation of ratiometric dadt. In this program, we will use the tools of supramolecular chemistry to synergistically engineer both the protein and AuNP quencher to provide highly efficient and versatile platforms for protein and cell surface sensing.

Public Health Relevance

Rapid and efficient sensing of proteins and cell surfaces will provide access to new diagnostics for many diseases.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
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Nanotechnology Study Section (NANO)
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Fabian, Miles
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University of Massachusetts Amherst
Schools of Arts and Sciences
United States
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Jeong, Youngdo; Kim, Sung Tae; Jiang, Ying et al. (2016) Nanoparticle-dendrimer hybrid nanocapsules for therapeutic delivery. Nanomedicine (Lond) 11:1571-8
Jiang, Ying; Wang, Ming; Hardie, Joseph et al. (2016) Chemically Engineered Nanoparticle-Protein Interface for Real-Time Cellular Oxidative Stress Monitoring. Small 12:3775-9
Mizuhara, Tsukasa; Moyano, Daniel F; Rotello, Vincent M (2016) Using the Power of Organic Synthesis for Engineering the Interactions of Nanoparticles with Biological Systems. Nano Today 11:31-40
Yoshii, Tatsuyuki; Geng, Yingying; Peyton, Shelly et al. (2016) Biochemical and biomechanical drivers of cancer cell metastasis, drug response and nanomedicine. Drug Discov Today 21:1489-94
Jiang, Ying; Huo, Shuaidong; Hardie, Joseph et al. (2016) Progress and perspective of inorganic nanoparticle-based siRNA delivery systems. Expert Opin Drug Deliv 13:547-59
Rana, Subinoy; Elci, S Gokhan; Mout, Rubul et al. (2016) Ratiometric Array of Conjugated Polymers-Fluorescent Protein Provides a Robust Mammalian Cell Sensor. J Am Chem Soc 138:4522-9
Tang, Rui; Jiang, Ziwen; Ray, Moumita et al. (2016) Cytosolic delivery of large proteins using nanoparticle-stabilized nanocapsules. Nanoscale 8:18038-18041
Chen, Juhong; Jackson, Angelyca A; Rotello, Vincent M et al. (2016) Colorimetric Detection of Escherichia coli Based on the Enzyme-Induced Metallization of Gold Nanorods. Small 12:2469-75
Hardie, Joseph; Jiang, Ying; Tetrault, Emily R et al. (2016) Simultaneous cytosolic delivery of a chemotherapeutic and siRNA using nanoparticle-stabilized nanocapsules. Nanotechnology 27:374001
Moyano, Daniel F; Liu, Yuanchang; Peer, Dan et al. (2016) Modulation of Immune Response Using Engineered Nanoparticle Surfaces. Small 12:76-82

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