The """"""""chemical nose/tongue"""""""" approach presents a potential alternative to specific recognition and separations techniques. In this approach a sensor array is generated to provide differential interaction with analytes via selective receptors, generating a stimulus response pattern that can be statistically analyzed and used for the identification of individual target analytes and also analysis of complex mixtures. Recently, we have developed nanoparticle-fluorescent polymer sensors for identification of proteins, bacteria, and cancerous cells through a fluorophore-displacement mechanism as well as a highly sensitive nanoparticle-GFP based """"""""chemical nose"""""""" strategy for protein detection in biofluid. Current chemical nose sensors for proteins and cell-surfaces are single channel, meaning a separate well or channel is required for each sensing element. This requirement for spatially distinct sensor elements complicates both microplate-based techniques and the application of array-based sensing in other venues, including microfluidics platforms and tissue staining. To overcome this limitation, we will exploit the spectral range of fluorescent proteins (FPs) to provide multi-channel fluorescence transduction for sensing applications. Multi-channel sensing will facilitate implementation of array-based sensing, allowing """"""""one well"""""""" sensing while improving sensitivity through generation of ratiometric dadt. In this program, we will use the tools of supramolecular chemistry to synergistically engineer both the protein and AuNP quencher to provide highly efficient and versatile platforms for protein and cell surface sensing.
Rapid and efficient sensing of proteins and cell surfaces will provide access to new diagnostics for many diseases.
|Gupta, Akash; Mumtaz, Shazia; Li, Cheng-Hsuan et al. (2018) Combatting antibiotic-resistant bacteria using nanomaterials. Chem Soc Rev :|
|Gupta, Akash; Das, Riddha; Yesilbag Tonga, Gulen et al. (2018) Charge-Switchable Nanozymes for Bioorthogonal Imaging of Biofilm-Associated Infections. ACS Nano 12:89-94|
|Kelly, Christopher; Tullius, Ryan; Lapthorn, Adrian J et al. (2018) Chiral Plasmonic Fields Probe Structural Order of Biointerfaces. J Am Chem Soc 140:8509-8517|
|Zhu, Dong Yu; Landis, Ryan F; Li, Cheng-Hsuan et al. (2018) Dynamically crosslinked polymer nanocomposites to treat multidrug-resistant bacterial biofilms. Nanoscale 10:18651-18656|
|Jiang, Ying; Hardie, Joseph; Liu, Yuanchang et al. (2018) Nanocapsule-mediated cytosolic siRNA delivery for anti-inflammatory treatment. J Control Release 283:235-240|
|Ray, Moumita; Lee, Yi-Wei; Hardie, Joseph et al. (2018) CRISPRed Macrophages for Cell-Based Cancer Immunotherapy. Bioconjug Chem 29:445-450|
|Landis, Ryan F; Li, Cheng-Hsuan; Gupta, Akash et al. (2018) Biodegradable Nanocomposite Antimicrobials for the Eradication of Multidrug-Resistant Bacterial Biofilms without Accumulated Resistance. J Am Chem Soc 140:6176-6182|
|Luther, D C; Lee, Y W; Nagaraj, H et al. (2018) Delivery approaches for CRISPR/Cas9 therapeutics in vivo: advances and challenges. Expert Opin Drug Deliv 15:905-913|
|Scaletti, Federica; Hardie, Joseph; Lee, Yi-Wei et al. (2018) Protein delivery into cells using inorganic nanoparticle-protein supramolecular assemblies. Chem Soc Rev 47:3421-3432|
|Le, Ngoc D B; Yesilbag Tonga, Gulen; Mout, Rubul et al. (2017) Cancer Cell Discrimination Using Host-Guest ""Doubled"" Arrays. J Am Chem Soc 139:8008-8012|
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