Embryonic cells polarize to develop specializations needed for morphogenesis and differentiation. Symmetry- breaking cues polarize many cells by altering RhoGTPase signaling, which triggers the asymmetric cortical localization of PAR polarity proteins. The molecular links that connect polarity cues, RhoGTPase signaling, and PAR asymmetries are poorly understood. Using the C. elegans embryo as a simple model, the long-term goal of this project is to determine how cell contact cues regulate RhoGTPases to induce the PAR asymmetries that polarize cells. Our ability to combine embryological manipulations with cell biological and genetic tools provides a unique opportunity to identify and characterize these mechanisms in living embryos. Given the deep conservation of cell polarity pathways and regulators, our studies will provide new insights into mechanisms of critical contact-mediated polarization events in humans. These include the polarization of embryonic blastomeres that occurs during compaction and is required for specification of the inner cell mass (which gives rise to the embryo proper and is the source of embryonic stem cells);and the polarization of epithelial cells that is needed for organogenesis and is essential for inhibiting tumor formation and invasion. In the prior award period, we defined a molecular pathway that mediates the contact-induced polarization of blastomeres by spatially altering the activity of the RhoGTPase CDC-42 - a signaling protein with an ancient and broadly conserved role in cell polarity. We showed that cell contacts recruit the conserved RhoGAP protein PAC-1/ARHGAP10, which inactivates CDC-42 at contact sites. CDC-42 remains active at contact-free surfaces, where it recruits the PAR proteins PAR-3, PAR-6, and PKC-3/aPKC that then polarize each blastomere. In addition, we have obtained preliminary data suggesting that CDC-42 controls the localization of PAR-6 and PAR-3 through distinct mechanisms, and that CDC-42 localizes PAR-3 by regulating membrane trafficking. The goal of this proposal is to identify the molecular links between cell-cell contact, PAC-1 recruitment, and CDC-42 activity that lead to PAR-3 asymmetry. Our specific goals are (1) to determine how cell contacts between blastomeres recruit PAC-1 to locally inactivate CDC-42;(2) to identify the mechanisms that activate CDC-42 at contact-free surfaces;and (3) to test the hypothesis that CDC-42 controls PAR-3 asymmetry by regulating membrane trafficking. Our experiments will allow us to build a molecular pathway that connects cell-cell contact to CDC-42 activity and its regulation of polarity. We anticipate that our findings will provide general insights into the fundamental mechanisms of cell polarization, and specific molecular insights into how cell contacts polarize human blastomeres and epithelial cells to promote embryonic development and inhibit tumorigenesis.

Public Health Relevance

We are using a simple genetic model system to learn how contacts between embryonic cells induce them to develop polarities that are essential for their function. Our findings will help in understanding how cell polarization occurs during normal human development, and how the loss of polarity in human cells can lead to tumor formation and invasion.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM078341-06
Application #
8292728
Study Section
Development - 2 Study Section (DEV2)
Program Officer
Hoodbhoy, Tanya
Project Start
2007-05-01
Project End
2016-04-30
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
6
Fiscal Year
2012
Total Cost
$338,000
Indirect Cost
$138,000
Name
New York University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
121911077
City
New York
State
NY
Country
United States
Zip Code
10016
Nance, Jeremy (2014) Getting to know your neighbor: cell polarization in early embryos. J Cell Biol 206:823-32
Armenti, Stephen T; Chan, Emily; Nance, Jeremy (2014) Polarized exocyst-mediated vesicle fusion directs intracellular lumenogenesis within the C. elegans excretory cell. Dev Biol 394:110-21
Chan, Emily; Nance, Jeremy (2013) Mechanisms of CDC-42 activation during contact-induced cell polarization. J Cell Sci 126:1692-702
Nance, Jeremy; Zallen, Jennifer A (2011) Elaborating polarity: PAR proteins and the cytoskeleton. Development 138:799-809
Wehman, Ann M; Poggioli, Corey; Schweinsberg, Peter et al. (2011) The P4-ATPase TAT-5 inhibits the budding of extracellular vesicles in C. elegans embryos. Curr Biol 21:1951-9
Achilleos, Annita; Wehman, Ann M; Nance, Jeremy (2010) PAR-3 mediates the initial clustering and apical localization of junction and polarity proteins during C. elegans intestinal epithelial cell polarization. Development 137:1833-42
Rohrschneider, Monica R; Nance, Jeremy (2009) Polarity and cell fate specification in the control of Caenorhabditis elegans gastrulation. Dev Dyn 238:789-96
Anderson, Dorian C; Gill, Jason S; Cinalli, Ryan M et al. (2008) Polarization of the C. elegans embryo by RhoGAP-mediated exclusion of PAR-6 from cell contacts. Science 320:1771-4