Probiotics are live organisms that confer a benefit to their host in some fashion. Bacteroides fragilis is one such probiotic by virtue of the immunomodulatory properties of its capsular polysaccharide PSA, the founding member of a novel class of MHC class II-presented carbohydrate T cell antigens (glycoantigens). Oral exposure to PSA in gnotobiotic mice restores the Th1/Th2 balance and immune homeostasis while rendering these animals less susceptible to inflammatory diseases through the induction of regulatory T cells. Our published and preliminary data further demonstrate that the nature of the N-linked glycans decorating antigen presenting cells, and specifically MHCII, is a critical aspect of the mechanism by which glycoantigens are presented and recognized by T cells. These innovative and unexpected findings suggest that immune homeostasis could be regulated by cellular glycosylation by virtue of the impact host glycans have on the induction of commensal-specific Treg cells. Here, we propose three specific aims to obtain a complete mechanistic and structural understanding of how host glycosylation modulates glycoantigen presentation, peripheral inflammation, and ultimately immune homeostasis at the molecular, cellular, and organismal levels. The results from our proposed experiments will reveal regulatory connections between inflammation and glycosylation through carbohydrate antigen activity and could lead to drug target identification to prevent and/or treat ongoing inflammation in diseases as diverse as asthma, IBD, atherosclerosis, and cancer.
Our findings during the previous funding period show there is interplay between protein glycosylation, protective anti-inflammatory immune responses, and the prevention of inflammatory diseases by commensal bacteria-derived polysaccharide antigens. This proposal seeks to provide an in depth analysis of the mechanism underlying this interplay in order to understand and manipulate these relationships to improve the outcome for patients with diseases where underlying inflammation is a causative force for disease progression and pathology.
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|Jones, Mark B; Oswald, Douglas M; Joshi, Smita et al. (2016) B-cell-independent sialylation of IgG. Proc Natl Acad Sci U S A 113:7207-12|
|Johnson, Jenny L; Jones, Mark B; Cobb, Brian A (2015) Polysaccharide A from the capsule of Bacteroides fragilis induces clonal CD4+ T cell expansion. J Biol Chem 290:5007-14|
|Johnson, Jenny L; Jones, Mark B; Cobb, Brian A (2015) Bacterial capsular polysaccharide prevents the onset of asthma through T-cell activation. Glycobiology 25:368-75|
|Taylor, Patricia R; Roy, Sanhita; Leal Jr, Sixto M et al. (2014) Activation of neutrophils by autocrine IL-17A-IL-17RC interactions during fungal infection is regulated by IL-6, IL-23, ROR?t and dectin-2. Nat Immunol 15:143-51|
|Ryan, Sean O; Abbott, Derek W; Cobb, Brian A (2014) Myeloid glycosylation defects lead to a spontaneous common variable immunodeficiency-like condition with associated hemolytic anemia and antilymphocyte autoimmunity. J Immunol 192:5561-70|
|Ryan, Sean O; Leal Jr, Sixto M; Abbott, Derek W et al. (2014) Mgat2 ablation in the myeloid lineage leads to defective glycoantigen T cell responses. Glycobiology 24:262-71|
|Ryan, Sean O; Johnson, Jenny L; Cobb, Brian A (2013) Neutrophils confer T cell resistance to myeloid-derived suppressor cell-mediated suppression to promote chronic inflammation. J Immunol 190:5037-47|
|Johnson, Jenny L; Jones, Mark B; Ryan, Sean O et al. (2013) The regulatory power of glycans and their binding partners in immunity. Trends Immunol 34:290-8|
|Bloem, Karien; García-Vallejo, Juan J; Vuist, Ilona M et al. (2013) Interaction of the Capsular Polysaccharide A from Bacteroides fragilis with DC-SIGN on Human Dendritic Cells is Necessary for Its Processing and Presentation to T Cells. Front Immunol 4:103|
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