The ATP synthase is a membrane-bound, energy-coupling rotary motor that is responsible for the synthesis of most cellular ATP in animals, plants and many bacteria. It consists of two sub-complexes with distinct, partial functions: the FO complex contains transmembrane subunits and functions in the transport of protons;the F1 is a peripheral complex, which contains the catalytic nucleotide binding sites for ATP synthesis. FO and F1 are coupled through two stalk-like connections of subunits: a central rotor shaft and a peripheral stator. In vitro, F1 can be dissociated from FO as a water-soluble enzyme that only catalyzes net hydrolysis of ATP, and this also serves as a simple sub-system for studying much of the enzymatic features of the enzyme. General features of catalysis by F1 and intact ATP synthase (FOF1) are shared among all types of the enzyme (mitochondrial, chloroplast and bacterial), but some factors that regulate its function appear to be adapted or unique to the metabolic demands of the specific organism. For example, mounting evidence indicates that in E.coli conformational changes in ?'s C-terminal domain (CTD) play one or more roles in regulating the activity and/or efficiency of energy coupling in the ATP synthase of bacteria and chloroplasts. The long-term goal of this project aims to gain a detailed understanding of the E. coli ATP synthase structure, function and regulation and thus provide a quantitative experimental model system to understand common structural/functional features of this important enzyme `family'. To achieve this goal we will focus our research in two directions:
In Aim 1, we will carry out high-resolution crystallographic studies on the E. coli F1-ATPase. This structural work is aimed at providing atomic snapshots the EcF1-? with the ? subunit in an extended (open) and closed conformation. We hypothesize that these two structural conformations, respectively, best describe the inhibitory and activating conformations adopted by the ? subunit, which is a critical regulator of E. coli FOF1 ATP synthase.
In Aim 2 we will study the dynamic nature of the ? subunit's inhibitory action on E.coli F1 and FOF1. Using ? mutants and disulfide crosslinking within ? we will restrict or bias the possible conformational states of ?'s CTD, in order to better define the regulatory roles of ? in EcF1 and EcFOF1, and which conformations of ?'s CTD are necessary for these roles. In addition, we will explore the possibility of using peptides spanning all or part of the ?-CTD to inhibit, in trans, the activity of F1 or FOF1. This will guide the rational basis for design of small molecules that selectively inhibit bacterial ATP synthases and not the mitochondrial enzyme.

Public Health Relevance

The ATP synthase is a central enzyme in cellular metabolism - it is responsible for making most ATP, the primary energy currency used inside cells. Defects in its assembly or function can cause human diseases and aggravate harmful events such as cardiac ischemia. Understanding unique features of the bacterial ATP synthase may lead to new approaches to fight infections.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM083088-04
Application #
8208225
Study Section
Biochemistry and Biophysics of Membranes Study Section (BBM)
Program Officer
Anderson, Vernon
Project Start
2008-12-01
Project End
2014-11-30
Budget Start
2011-12-01
Budget End
2014-11-30
Support Year
4
Fiscal Year
2012
Total Cost
$431,025
Indirect Cost
$144,667
Name
Upstate Medical University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
058889106
City
Syracuse
State
NY
Country
United States
Zip Code
13210
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Shah, Naman B; Duncan, Thomas M (2014) Bio-layer interferometry for measuring kinetics of protein-protein interactions and allosteric ligand effects. J Vis Exp :e51383
Duncan, Thomas M; Düser, Monika G; Heitkamp, Thomas et al. (2014) Regulatory conformational changes of the ? subunit in single FRET-labeled FoF1-ATP synthase. Proc SPIE Int Soc Opt Eng 8948:89481J
Bockenhauer, Samuel D; Duncan, Thomas M; Moerner, W E et al. (2014) The regulatory switch of F1-ATPase studied by single-molecule FRET in the ABEL Trap. Proc SPIE Int Soc Opt Eng 8950:89500H
Borsch, Michael; Duncan, Thomas M (2013) Spotlighting motors and controls of single FoF1-ATP synthase. Biochem Soc Trans 41:1219-26
Shah, Naman B; Hutcheon, Marcus L; Haarer, Brian K et al. (2013) F1-ATPase of Escherichia coli: the ýý- inhibited state forms after ATP hydrolysis, is distinct from the ADP-inhibited state, and responds dynamically to catalytic site ligands. J Biol Chem 288:9383-95
Cingolani, Gino; Duncan, Thomas M (2011) Structure of the ATP synthase catalytic complex (F(1)) from Escherichia coli in an autoinhibited conformation. Nat Struct Mol Biol 18:701-7