The long-term goal of my laboratory is understanding the molecular mechanism(s) by which maternal contribution shapes the early development of an embryo, and how the transition to zygotic control is carried out. This proposal focuses on maternal factors controlling the development of germline precursors in early C. elegans embryos. Germ cell precursors, like stem cells, are maintained transcriptionally inactive in a wide variety of animals. In C. elegans, germ cell precursors are generated through four rounds of asymmetric stem cell-like divisions, beginning with the 1-cell zygote, P0. Each division gives rise to a smaller transcriptionally repressed germline blastomere (P1-P4), and a larger somatic sister cell which soon becomes transcriptionally active. This transcriptional repression has been shown to require a maternally- supplied protein, PIE-1. Although PIE-1 protein is in all germline blastomeres, it does not account for repression in P0 or P1. We show that two closely related maternal proteins, OMA-1 and -2, are required for transcriptional repression in all germline blastomeres, through a mechanism distinct from PIE-1 repression. OMA-1 and OMA-2 are expressed only in oocytes and 1-cell embryos, and their degradation after the 1-cell stage requires developmentally-regulated phosphorylation events. OMA-1 and OMA-2 repress transcription directly in 1-cell and early 2-cell embryos by sequestering an essential component of the basal transcription complex, TAF-4, in the cytoplasm. OMA proteins do not sequester TAF-4 to the cytoplasm in oocytes. In addition, we propose that OMA-1/2 prevent PIE-1 from being degraded in the gonad, thereby indirectly maintaining transcriptional repression in later germline blastomeres. Our data are consistent with OMA-1/2 preventing PIE-1 degradation through translational repression of a factor regulating PIE-1 degradation.
Aim 1 investigates the relationship between regulation of OMA-1 degradation and TAF-4 binding. Surprisingly, the same phosphorylation event that earmarks OMA-1 for degradation is required for TAF-4 binding.
Aim 2 investigates the mechanism by which OMA proteins maintain PIE-1 stability in oocytes. OMA-1 and -2 belong to a protein family with RNA- binding activity, and preliminary results support OMA binding to the mRNA for a protein critical for PIE-1 degradation and inhibiting its translation specifically in oocytes.
Aim 3 investigates how the phosphorylation events that occur following fertilization convert OMA function from stabilizing PIE- 1 protein in oocytes via translational inhibition to regulating germ cell precursor development in the early embryo by transcriptional repression. Insights gained should be relevant to our understanding of the oocyte-to-embryo transition, germ cell development and stem cell biology.

Public Health Relevance

One of the most exciting areas of molecular medicine, and one with potentially immediate and broad impact upon public health, is that of stem cells, and in particular adult stem cells. As research on these various stem cell populations, including germ cells, continues, it appears that many, if not all of these cells, may retain pluripotency, even possibly totipotency. Our studies address the question of how germ cell precursors are maintained totipotent in the early embryo, and any insights gained from our experimental system are expected to shed light on the regulation of stem cell populations in general.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM084198-03
Application #
8106447
Study Section
Development - 1 Study Section (DEV1)
Program Officer
Haynes, Susan R
Project Start
2009-08-01
Project End
2013-07-31
Budget Start
2011-08-01
Budget End
2012-07-31
Support Year
3
Fiscal Year
2011
Total Cost
$307,751
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Biochemistry
Type
Schools of Medicine
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
Martin, Adam; Rex, Emily A; Ishidate, Takao et al. (2017) Infection of Caenorhabditis elegans with Vesicular Stomatitis Virus via Microinjection. Bio Protoc 7:
Robertson, Scott M; Medina, Jessica; Oldenbroek, Marieke et al. (2017) Reciprocal signaling by Wnt and Notch specifies a muscle precursor in the C. elegans embryo. Development 144:419-429
Yang, Xiao-Dong; Karhadkar, Tejas R; Medina, Jessica et al. (2015) ?-Catenin-related protein WRM-1 is a multifunctional regulatory subunit of the LIT-1 MAPK complex. Proc Natl Acad Sci U S A 112:E137-46
Robertson, Scott; Lin, Rueyling (2015) The Maternal-to-Zygotic Transition in C. elegans. Curr Top Dev Biol 113:1-42
Robertson, Scott M; Medina, Jessica; Lin, Rueyling (2014) Uncoupling different characteristics of the C. elegans E lineage from differentiation of intestinal markers. PLoS One 9:e106309
Spike, Caroline A; Coetzee, Donna; Nishi, Yuichi et al. (2014) Translational control of the oogenic program by components of OMA ribonucleoprotein particles in Caenorhabditis elegans. Genetics 198:1513-33
Robertson, Scott; Lin, Rueyling (2013) The oocyte-to-embryo transition. Adv Exp Med Biol 757:351-72
Oldenbroek, Marieke; Robertson, Scott M; Guven-Ozkan, Tugba et al. (2013) Regulation of maternal Wnt mRNA translation in C. elegans embryos. Development 140:4614-23
Oldenbroek, Marieke; Robertson, Scott M; Guven-Ozkan, Tugba et al. (2012) Multiple RNA-binding proteins function combinatorially to control the soma-restricted expression pattern of the E3 ligase subunit ZIF-1. Dev Biol 363:388-98
Yang, Xiao-Dong; Huang, Shuyi; Lo, Miao-Chia et al. (2011) Distinct and mutually inhibitory binding by two divergent ?-catenins coordinates TCF levels and activity in C. elegans. Development 138:4255-65

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