The role of the liver during the post-burn response is essentially unknown, and many burn and trauma surgeons consider the liver as unimportant, with little or no effect on post-burn outcomes. Others strongly suggest that intact liver function and integrity are essential for burn patients'recovery, because the liver modulates metabolic pathways, inflammatory processes, immune functions, and acute-phase responses. Our preliminary data indicate that a severe thermal injury causes hepatic damage and dysfunction by inducing hepatocyte endoplasmic reticulum stress (ER stress) and hepatocyte apoptosis. We hypothesize that hepatic ER stress and subsequent apoptosis contribute to adverse post-burn outcomes, and that attenuating hepatic ER stress and apoptosis will enhance hepatic function, which is associated with improved post-burn morbidity and mortality. The exact mechanisms by which a burn induces hepatic ER stress, apoptosis, and dysfunction are not known, limiting therapeutic intervention. In preliminary studies we found that these pathophysiological processes are related to hepatic inflammation, altered phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling, depletion of the hepatic calcium stores, the unfolded protein response (UPR), and mitochondrial dysfunction. We now propose to characterize the post-burn molecular and physiological changes that lead to hepatic apoptosis and dysfunction. Hypothesis 2 is that burn induces hepatic damage by altering the PI3K/Akt and inositol 1, 4, 5,-triphosphate receptor (IP3R) signaling pathways, leading to calcium store depletion, cytochrome c release, ER stress/UPR, and consequently to hepatocyte apoptosis.
In Aim 1 we will determine whether post-burn hepatocyte ER stress and apoptosis contribute to morbidity and mortality.
Aim 2 is to determine in vivo the cellular and sub-cellular mechanisms which cause post-burn alterations in hepatic intracellular calcium, ER stress and UPR, and hepatocyte apoptosis.
Aim 3 is designed to identify and characterize an intervention that will improve hepatic integrity and function post-burn and is readily available in the clinical setting. Based on our preliminary data we will use insulin, which attenuates hepatic damage and improves liver function post-burn. The mechanisms underlying these effects are not understood;we hypothesize that post-burn insulin administration improves hepatocyte viability and liver function via normalization of intracellular calcium and IP3R signaling pathways, attenuation of cytochrome c release, and reduction of ER stress/UPR, leading to markedly decreased hepatocyte apoptosis. We further hypothesize that insulin exerts its effects via insulin-specific signal transduction pathway activation. We will test this hypothesis by comparing the effects of insulin on hepatocyte ER stress/UPR and apoptosis to the effects of a glucose- lowering drug, as well as chemical ER chaperones on ER stress/UPR, apoptosis, and post-burn outcomes. Identifying and understanding the cellular and molecular mechanisms by which insulin or glucose modulation will improve outcomes could lead to the establishment of novel treatment options for severely burned patients.
A key and novel aspect of this study is our hypothesis that hepatic apoptosis and ER stress play an important role in post-burn morbidity and mortality, and that insulin administration improves these adverse outcomes by decreasing hepatic apoptosis and ER stress. This study is important because characterizing and identifying the molecular mechanisms associated with burn-induced hepatic apoptosis and ER stress will lead to improvements in the clinical care of severely burned patients.
|Banfield, Joanne; Rehou, Sarah; Gomez, Manuel et al. (2015) Healthcare costs of burn patients from homes without fire sprinklers. J Burn Care Res 36:213-7|
|Austin, Ryan E; Shahrokhi, Shahriar; Bolourani, Siavash et al. (2015) Peripherally inserted central venous catheter safety in burn care: a single-center retrospective cohort review. J Burn Care Res 36:111-7|
|Kraft, Robert; Herndon, David N; Finnerty, Celeste C et al. (2015) Predictive Value of IL-8 for Sepsis and Severe Infections After Burn Injury: A Clinical Study. Shock 43:222-7|
|Austin, Ryan E; Merchant, Nishant; Shahrokhi, Shahriar et al. (2015) A comparison of Biobrane™ and cadaveric allograft for temporizing the acute burn wound: Cost and procedural time. Burns 41:749-53|
|Arno, Anna I; Gauglitz, Gerd G; Barret, Juan P et al. (2014) New molecular medicine-based scar management strategies. Burns 40:539-51|
|Jeschke, Marc G; Pinto, Ruxandra; Herndon, David N et al. (2014) Hypoglycemia is associated with increased postburn morbidity and mortality in pediatric patients. Crit Care Med 42:1221-31|
|Diao, Li; Marshall, Alexandra H; Dai, Xiaojing et al. (2014) Burn plus lipopolysaccharide augments endoplasmic reticulum stress and NLRP3 inflammasome activation and reduces PGC-1? in liver. Shock 41:138-44|
|Shahrokhi, Shahriar; Arno, Anna; Jeschke, Marc G (2014) The use of dermal substitutes in burn surgery: acute phase. Wound Repair Regen 22:14-22|
|Arno, Anna I; Gauglitz, Gerd G; Barret, Juan P et al. (2014) Up-to-date approach to manage keloids and hypertrophic scars: a useful guide. Burns 40:1255-66|
|Jeschke, Marc G; Gauglitz, Gerd G; Finnerty, Celeste C et al. (2014) Survivors versus nonsurvivors postburn: differences in inflammatory and hypermetabolic trajectories. Ann Surg 259:814-23|
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