A eukaryotic cell must be able to transport macromolecules directionally between its nucleus and cytoplasm, and to divide the cell through mitosis. These fundamental processes are controlled by localizing the small Ran guanosine triphosphatase (GTPase) protein in its GDP or GTP bound state within the cytoplasm or the nucleus respectively, and by generating a gradient of RanGTP around the chromosomes. The spatial localization of RanGTP in the nucleus is achieved through chromatin bound RCC1 (regulator of chromosomal condensation) protein. RCC1 recruits Ran to the chromosomes and promotes the exchange of RanGDP for RanGTP, thereby creating a high concentration of RanGTP around chromosomes. We currently lack a molecular understanding of how RCC1 binds to the nucleosome and how RCC1 recruits Ran to the nucleosome, despite the critical importance of these interactions for basic cellular processes. Our overall goal is therefore to develop atomic models which describe how RCC1 and Ran bind to the nucleosome core particle.
Our specific aims are: 1. Define how RCC1 binds to nucleosomes through biochemical methods. We will challenge structural models for how RCC1 interacts with the nucleosome through pulldown, biolayer interferometry and fluorescence resonance energy transfer experiments. 2. Determine the structure of the RCC1/nucleosome complex. We will use single crystals of the RCC1/nucleosome complex we have grown to determine the structure of the complex. These crystallographic studies will be complemented with small angle X-ray and neutron scattering experiments to provide a solution structure of the complex. 3. Determine how chromatin-bound RCC1 binds to and activates Ran. We will test models for the Ran/RCC1/nucleosome complex by analyzing the effects of directed mutations on binding of Ran to the RCC1/nucleosome complex and on Ran's nucleotide exchange activity in the presence of RCC1 and the nucleosome.
When a cell divides, each daughter cell must receive an equal share of the chromosomes which carry the cell's genetic blueprint. Unequal or improper distribution of the chromosomes can result in genetic instabilities and cancer. Our studies are directed at visualizing the molecules which regulate the equal distribution of chromosomes during cell division by creating a GPS or genome-positioning system for a eukaryotic cell.
|McGinty, Robert K; Tan, Song (2016) Recognition of the nucleosome by chromatin factors and enzymes. Curr Opin Struct Biol 37:54-61|
|Girish, Taverekere S; McGinty, Robert K; Tan, Song (2016) Multivalent Interactions by the Set8 Histone Methyltransferase With Its Nucleosome Substrate. J Mol Biol 428:1531-43|
|Jennings, Matthew J; Barrios, Adam F; Tan, Song (2016) Elimination of truncated recombinant protein expressed in Escherichia coli by removing cryptic translation initiation site. Protein Expr Purif 121:17-21|
|Liokatis, Stamatios; Klingberg, Rebecca; Tan, Song et al. (2016) Differentially Isotope-Labeled Nucleosomes To Study Asymmetric Histone Modification Crosstalk by Time-Resolved NMR Spectroscopy. Angew Chem Int Ed Engl 55:8262-5|
|Kuo, Yin-Ming; Henry, Ryan A; Tan, Song et al. (2015) Site specificity analysis of Piccolo NuA4-mediated acetylation for different histone complexes. Biochem J 472:239-48|
|Kim, Sang-Ah; Chatterjee, Nilanjana; Jennings, Matthew J et al. (2015) Extranucleosomal DNA enhances the activity of the LSD1/CoREST histone demethylase complex. Nucleic Acids Res 43:4868-80|
|McGinty, Robert K; Tan, Song (2015) Nucleosome structure and function. Chem Rev 115:2255-73|
|McGinty, Robert K; Henrici, Ryan C; Tan, Song (2014) Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome. Nature 514:591-6|
|Makde, Ravindra D; Tan, Song (2013) Strategies for crystallizing a chromatin protein in complex with the nucleosome core particle. Anal Biochem 442:138-45|
|Tan, Song; Nagai, Kiyoshi (2013) Protein-nucleic interactions: 'I have a cunning planâ€¦'. Curr Opin Struct Biol 23:90-2|
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