Recent technological advances have led to the isolation of multiple pluripotent cell lines derived from embryonic and adult tissues and from multiple mammalian species. These new sources of pluripotent cells provide novel tools that offer multiple rewards for basic and clinical research.
The aim of this study is to create a novel strategy to generate rodents derived solely from pluripotent cells. Our proposal is based on the creation of mosaic embryos composed of pluripotent cells and genetically defective embryos. Using this strategy, we force the generation of animals composed solely of the donor pluripotent cells. This strategy will facilitate the generation of mice and rats to model human disease. It will also advance our understanding of mammalian gene function and the identification of gene products of therapeutic value.

Public Health Relevance

The study proposes a new strategy for rodent cloning using pluripotent cells. This new technology will facilitate the generation of transgenic mice and rats models for human disease. In addition, it will provide genetic tools for the functional annotation of the mouse genome, an enterprise with the potential to elucidate gene function and identification of gene products with therapeutic value.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM094874-03
Application #
8326633
Study Section
Special Emphasis Panel (ZGM1-GDB-7 (EU))
Program Officer
Haynes, Susan R
Project Start
2010-09-01
Project End
2014-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
3
Fiscal Year
2012
Total Cost
$325,710
Indirect Cost
$127,710
Name
University of Massachusetts Medical School Worcester
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
01655
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Follit, John A; San Agustin, Jovenal T; Jonassen, Julie A et al. (2014) Arf4 is required for Mammalian development but dispensable for ciliary assembly. PLoS Genet 10:e1004170