Protein drugs have proven themselves as highly important therapeutic drugs in treating various diseases, including cancer and cardiovascular diseases. The benefits of various antibodies for treating cancers are well known. One of the major problems with protein drugs is their short half life in blood circulation. The drug delivery formations that can deliver protein drugs for long-term, ranging from weeks to months, will provide a basic tool for delivering a variety of protein drugs, enabling the sustained and powerful impact to the field. Particulate formulations in the micro-sizes (""""""""microparticles"""""""") have been extensively studied for long-term delivery of low molecular weight drugs, but their success with protein drugs has been extremely limited. The deficiencies of the current particulate formulations prepared by the emulsion methods include heterogeneous particle size, low drug loading capacity, high initial burst release, incomplete drug release, and difficulty in scaling-up production. The goal of this research is to use the recently developed hydrogel template approach to formulate homogeneous microparticles for long-term (1 month ~ 3 months) delivery of protein drugs for clinical applications. The hypothesis in this proposal is that the hydrogel template-based fabrication provides particles of high drug loading with predictable release profiles by confining the protein/PLGA mixture to the micro wells in a hydrogel template during the microencapsulation process.
The specific aims of this project are: (i) to fabricate homogeneous nan0/micro structures using hydrogel templates;(ii) to characterize protein loading and release properties of nan0/micro structures;and (iii) to evaluate therapeutic effects by in vivo animal study and in vitro cell culture method. The innovation in the hydrogel template approach is that microparticles, which are fabricated inside individual wells of the hydrogel template, can be harvested by simply dissolving the template in water. Currently, no other fabrication methods allow such an easy way of harvesting the formed microparticles. The simplicity in the method allows easy scale-up production for clinical applications. The significance of this research is that the hydrogel template approach provides a new general method of precise fabrication of microparticles with predefined properties for protein drug delivery. The hydrogel template method is an enabling technique that can be applied to all types of drugs, making it easy to develop clinically useful formulations for various protein drugs.

Public Health Relevance

Protein drugs have been essential in treating various diseases, and yet the long-term delivery ranging from 1 month to 3 months has not been easy. The goal of this project is to use the newly developed hydrogel template-based nanofabrication methodology to prepare microparticles for more efficient long-term delivery of genetically engineered protein drugs.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM095879-02
Application #
8435333
Study Section
Special Emphasis Panel (ZRG1-SBIB-Q (02))
Program Officer
Somers, Scott D
Project Start
2012-03-01
Project End
2015-12-31
Budget Start
2013-01-01
Budget End
2013-12-31
Support Year
2
Fiscal Year
2013
Total Cost
$280,719
Indirect Cost
$92,544
Name
Purdue University
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Ma, Yanni; He, Shaolong; Ma, Xueqin et al. (2016) Silymarin-Loaded Nanoparticles Based on Stearic Acid-Modified Bletilla striata Polysaccharide for Hepatic Targeting. Molecules 21:265
Lee, Byung Kook; Yun, Yeonhee; Park, Kinam (2016) PLA micro- and nano-particles. Adv Drug Deliv Rev 107:176-191
Park, Kinam (2015) Drug delivery of the future: Chasing the invisible gorilla. J Control Release :
Wang, Hong; Zhang, Guangxing; Sui, Hong et al. (2015) Comparative studies on the properties of glycyrrhetinic acid-loaded PLGA microparticles prepared by emulsion and template methods. Int J Pharm 496:723-31
Yun, Yeon Hee; Lee, Byung Kook; Park, Kinam (2015) Controlled Drug Delivery: Historical perspective for the next generation. J Control Release 219:2-7
Lu, Ying; Wang, Zhao-hui; Li, Tonglei et al. (2014) Development and evaluation of transferrin-stabilized paclitaxel nanocrystal formulation. J Control Release 176:76-85
Park, Kinam (2014) Controlled drug delivery systems: past forward and future back. J Control Release 190:3-8
Lu, Ying; Sturek, Michael; Park, Kinam (2014) Microparticles produced by the hydrogel template method for sustained drug delivery. Int J Pharm 461:258-69
Shin, Crystal S; Kwak, Bongseop; Han, Bumsoo et al. (2013) Development of an in vitro 3D tumor model to study therapeutic efficiency of an anticancer drug. Mol Pharm 10:2167-75
Lee, Seung-Young; Kim, Sungwon; Tyler, Jacqueline Y et al. (2013) Blood-stable, tumor-adaptable disulfide bonded mPEG-(Cys)4-PDLLA micelles for chemotherapy. Biomaterials 34:552-61

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