The overall objective is to test the hypothesis that initial pathogen reproduction at the outbreak focus is the primary determinant of subsequent epidemic spread for pathogens with "fat-tailed" dispersal kernels, which have potential for significant long-distance dispersal (LDD). This will be accomplished by addressing three specific sub-hypotheses to test how the frequency of susceptible hosts, degree of host susceptibility, and application of fungicide at the outbreak focus influence subsequent epidemic spread. Methods: The wheat stripe rust disease will be used as a model system in two experiments conducted over four years. In both experiments, the artificially inoculated area ("outbreak focus") at the center of each experimental plot will be treated either the same or different from the remainder of the plot to determine whether initial conditions at plot center have the dominant influence over subsequent disease spread. In the first experiment, three host genetic variables that influence pathogen reproduction (monoculture of a susceptible host genotype, a mixture of 25% of the susceptible genotype with 75% of a resistant genotype, and a partially resistant genotype) will be planted in varying combinations of plot center and the remainder of the plot. In the second experiment, all plots will be planted to a monoculture of the susceptible genotype, and a highly effective fungicide will be used to curtail or "cull" disease at varying times after the beginning of the first infectious period. This will be done either in the outbreak focus only or in an area including both the outbreak focus plus three additional focus widths on each side of the artificially inoculated area. For both experiments, disease prevalence (% of maximum number of infections in each sampling unit) will be recorded weekly in the outbreak focus and at varying distances both downwind and upwind of the outbreak focus. Generality of the results will be evaluated by altering variables in a spatially explicit simulation model, by evaluating relevant data of other diseases in the literature, and through a collaboration to compare field and modeling results for wheat stripe rust with those of foot-and-mouth disease of livestock.

Public Health Relevance

The project has significant potential to increase our knowledge of the spread of LDD pathogens of taxonomically divergent hosts, and may lead to more ecologically-based methods to control major diseases and to mitigate invasive epidemics. Conclusions should apply over a very wide range of spatial scale due to the power law nature of the dispersal kernel for many LDD pathogens.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM096685-03
Application #
8309076
Study Section
Special Emphasis Panel (ZRG1-BDA-A (80))
Program Officer
Eckstrand, Irene A
Project Start
2010-09-30
Project End
2014-07-31
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
3
Fiscal Year
2012
Total Cost
$209,512
Indirect Cost
$66,207
Name
Oregon State University
Department
Other Basic Sciences
Type
Schools of Arts and Sciences
DUNS #
053599908
City
Corvallis
State
OR
Country
United States
Zip Code
97339
Mundt, Christopher C (2014) Durable resistance: a key to sustainable management of pathogens and pests. Infect Genet Evol 27:446-55
Severns, Paul M; Estep, Laura K; Sackett, Kathryn E et al. (2014) Degree of host susceptibility in the initial disease outbreak influences subsequent epidemic spread. J Appl Ecol 51:1622-1630
Mundt, Christopher C; Wallace, Larae D; Allen, Tom W et al. (2013) Initial epidemic area is strongly associated with the yearly extent of soybean rust spread in North America. Biol Invasions 15:1431-1438