Manganese and the regulation of its acquisition and subsequent metabolism are important for normal cell function, and contribute to the success of bacteria in adapting to the diverse environments in which they occupy. Manganese can be a limiting nutrient, and thus manganese-dependent processes must be coordinated with availability of the metal. We are interested in understanding how Bradyrhizobium japonicum senses and acquires manganese, maintains homeostasis, and coordinates manganese-dependent activities with cellular levels. Preliminary evidence lead us to test the hypothesis that B. japonicum adapts to cellular manganese levels by novel mechanisms that differ substantially from that found in E. coli and other model organisms. B. japonicum belongs to the alpha-Proteobacteria, a diverse taxonomic group that includes numerous members that form close or intracellular associations with eukaryotic hosts in a symbiotic or pathogenic context. B. japonicum serves as a model organism to study related pathogens that are refractory to experimental analysis.
Three specific aims are proposed. (1) Elucidate the mechanisms that maintain manganese homeostasis. We identified a high affinity outer membrane channel necessary for Mn2+ uptake. Outer membrane selectivity for a divalent ion is unprecedented and reveals a bacterial strategy for nutrient acquisition not previously appreciated. We will elucidate the role of this channel in manganese homeostasis. Moreover, B. japonicum tolerates an unusually high external manganese concentration that is toxic to other bacteria. We will determine the basis of this tolerance towards the goal of understanding how this organism maintains homeostasis. (2) Elucidate manganese-responsive transcriptional control via the B. japonicum Mur protein. B. japonicum has adapted a global bacterial iron-responsive transcriptional repressor to function as a Mn2+ responsive regulator. We uncovered novel aspects of the Mur protein. Firstly, it binds Mn2+ at a site dissimilar to the presumed metal binding sites of Fur family proteins, and resolving this issue should give insight as to how it has adapted to be a Mn2+ sensor. Secondly, the demetallated form of Mur is active and controls a different regulon than the Mn2+-loaded protein. We will analyze this novel function. (3) Determine the molecular basis for the integration of iron and manganese metabolism. Preliminary evidence shows that B. japonicum senses and responds to a simultaneous deficiency in both iron and manganese at the level of transcription that is qualitatively different from individual deficiencies in each metal. We will focus initially on two operons involved in branched chain amino acid degradation to gain insight into this global response.
The ability of a bacterium to successfully infect and colonize its host requires it to adapt to that new environment, which includes the acquisition of nutrients that may be limiting. Bradyrhizobium japonicum is a model bacterium, but is taxonomically related to pathogens that are refractory to experimental analysis. We are studying novel mechanisms of manganese regulation and metabolic control in B. japonicum towards the end of understanding molecular strategies that bacteria employ for adaptation.
|Hohle, Thomas H; O'Brian, Mark R (2016) Metal-specific control of gene expression mediated by Bradyrhizobium japonicum Mur and Escherichia coli Fur is determined by the cellular context. Mol Microbiol 101:152-66|
|Sankari, Siva; O'Brian, Mark R (2016) The Bradyrhizobium japonicum Ferrous Iron Transporter FeoAB Is Required for Ferric Iron Utilization in Free Living Aerobic Cells and for Symbiosis. J Biol Chem 291:15653-62|
|Amarelle, Vanesa; Rosconi, Federico; LÃ¡zaro-MartÃnez, Juan Manuel et al. (2016) HmuS and HmuQ of Ensifer/Sinorhizobium meliloti degrade heme in vitro and participate in heme metabolism in vivo. Biometals 29:333-47|
|Sankari, Siva; O'Brian, Mark R (2016) Synthetic Lethality of the bfr and mbfA Genes Reveals a Functional Relationship between Iron Storage and Iron Export in Managing Stress Responses in Bradyrhizobium japonicum. PLoS One 11:e0157250|
|O'Brian, Mark R (2015) Perception and Homeostatic Control of Iron in the Rhizobia and Related Bacteria. Annu Rev Microbiol 69:229-45|
|Hohle, Thomas H; O'Brian, Mark R (2014) Magnesium-dependent processes are targets of bacterial manganese toxicity. Mol Microbiol 93:736-47|
|Sankari, Siva; O'Brian, Mark R (2014) A bacterial iron exporter for maintenance of iron homeostasis. J Biol Chem 289:16498-507|
|Jaggavarapu, Siddharth; O'Brian, Mark R (2014) Differential control of Bradyrhizobium japonicum iron stimulon genes through variable affinity of the iron response regulator (Irr) for target gene promoters and selective loss of activator function. Mol Microbiol 92:609-24|
|Hohle, Thomas H; O'Brian, Mark R (2012) Manganese is required for oxidative metabolism in unstressed Bradyrhizobium japonicum cells. Mol Microbiol 84:766-77|
|Escamilla-Hernandez, Rosalba; O'Brian, Mark R (2012) HmuP is a coactivator of Irr-dependent expression of heme utilization genes in Bradyrhizobium japonicum. J Bacteriol 194:3137-43|