Protein quality control mechanisms are required for normal cellular health to prevent disease and avoid premature senescence. Unique mutant proteins are substrates of these pathways, are recognized by protein quality control pathway components and typically degraded by the proteasome. Proteins with subtle missense mutations can retain function yet degradation by these pathways can underlie pathogenesis. The molecular and physical basis of protein quality control substrate recognition are poorly understood, especially for soluble cytosolic proteins. This application proposes to use a powerful multi disciplinary approach to define novel proteins in the protein quality control pathway and elucidate the physical and structural basis of substrate recognition.

Public Health Relevance

Protein quality control mechanisms are required for normal cellular health to prevent disease and avoid premature senescence. Heritable missense mutations produce large quantities of mutant proteins that are recognized as mutant and degraded by various mechanisms. Unique mutant protein substrates bearing subtle mutations but remaining functional are degraded by these pathways yet the physical basis of their recognition and detection are poorly understood, especially for soluble cytosolic proteins. This application proposes to use a powerful multi disciplinary approach to define novel proteins in the protein QC pathway and elucidate the basis of substrate recognition.

Agency
National Institute of Health (NIH)
Type
Research Project (R01)
Project #
5R01GM103369-03
Application #
8646931
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Deatherage, James F
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Pittsburgh
Department
Pharmacology
Type
Schools of Medicine
DUNS #
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Towheed, Atif; Markantone, Desiree M; Crain, Aaron T et al. (2014) Small mitochondrial-targeted RNAs modulate endogenous mitochondrial protein expression in vivo. Neurobiol Dis 69:15-22
Hrizo, Stacy L; Fisher, Isaac J; Long, Daniel R et al. (2013) Early mitochondrial dysfunction leads to altered redox chemistry underlying pathogenesis of TPI deficiency. Neurobiol Dis 54:289-96
Roland, Bartholomew P; Stuchul, Kimberly A; Larsen, Samantha B et al. (2013) Evidence of a triosephosphate isomerase non-catalytic function crucial to behavior and longevity. J Cell Sci 126:3151-8