The extensive variation in the amount of harm that pathogens cause their hosts has intrigued biologists for centuries, but there have been surprisingly few opportunities to test theoretical predictions in non-laboratory systems. We propose work on a tractable and well-studied wildlife-pathogen system, the house finch and its bacterial pathogen, Mycoplasma gallisepticum, in which we have already documented the independent evolution of increasing virulence since the emergence of the pathogen in two distinct parts of the host's range. We hypothesize two ecological conditions that underlie the observed patterns of increasing virulence evolution detected to date: 1) imperfect acquired host immunity, akin to vaccination, has selected for higher rates of within-host pathogen replication and associated higher virulence;and 2) higher contact rates, such as caused by anthropogenic feeding of birds, have resulted in increased virulence by minimizing the pathogen's risk of killing its host before it successfully transmits. The proposed work involves integration of theoretical virulence models with proposed experiments. First, formal within- and between-host models describing the two hypotheses will identify information needed for model refinement and validation;second, targeted experiments will quantify key parameters;and, third, these data will feed back to evaluate whether the models'assumed conditions explain observed field patterns. The empirical data will come from controlled infection and transmission experiments, which are unusually tractable in our study system, in order to characterize within- and between-host dynamics for partially immune versus immunologically naive hosts and at high and low between-host contact rates. Supplementing these model-focused experiments will be DNA.sequence and expression data from multiple field and experimental isolates of the bacteria, where our knowledge of gene function in M. gallisepticum will allow us to identify which genes may underiie the detected virulence changes. These data will serve as an independent test of the partial immunity hypothesis by addressing whether bacterial genes responsible for evading host immune systems are systematically changing in vivo and in vitro under selection due to acquired immunity, consistent with our hypothesis.

Public Health Relevance

'ons): Understanding the mechanisms that drive virulence evolution has far-reaching implications for public health measures such as imperfect vaccination that may inadvertently drive increases iri virulence for human pathogens. Our work will also shed light on the role of anthropogenic feeding and alterations of host contact rates via urbanization on the evolution of virulence in zoonotic pathogens.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Project (R01)
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Special Emphasis Panel (ZRG1)
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Janes, Daniel E
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Virginia Polytechnic Institute and State University
Schools of Arts and Sciences
United States
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Ley, David H; Hawley, Dana M; Geary, Steven J et al. (2016) House Finch (Haemorhous mexicanus) Conjunctivitis, and Mycoplasma spp. Isolated from North American Wild Birds, 1994-2015. J Wildl Dis 52:669-73
Dhondt, André A; Dhondt, Keila V; Hochachka, Wesley M (2015) Response of black-capped chickadees to house finch Mycoplasma gallisepticum. PLoS One 10:e0124820
Osnas, Erik E; Hurtado, Paul J; Dobson, Andrew P (2015) Evolution of pathogen virulence across space during an epidemic. Am Nat 185:332-42
Williams, P D; Dobson, A P; Dhondt, K V et al. (2014) Evidence of trade-offs shaping virulence evolution in an emerging wildlife pathogen. J Evol Biol 27:1271-8
Hawley, Dana M; Osnas, Erik E; Dobson, Andrew P et al. (2013) Parallel patterns of increased virulence in a recently emerged wildlife pathogen. PLoS Biol 11:e1001570