Reactive oxygen species (ROS) are a significant byproduct of oxidative protein folding in the endoplasmic reticulum (ER) lumen. Little is known about how cells prevent excessive oxidant accumulation and oxidant-induced damage from ER folding events. Our proposed research aims to illuminate these protective cellular mechanisms. Taking advantage of our ability to selectively create a hyper-oxidized ER, we have identified two novel redox pathways that manage ER-derived ROS. Disruption of either pathway results in a loss of cell viability upon induction of oxidative stress, suggesting that both systems are essential to maintain normal ROS physiology. Our data indicate that each pathway is unique in the means by which it influences ROS dynamics. This proposal focuses on elucidating the mechanistic features of these two important and uncharacterized cellular redox systems.
In Aim 1, we will characterize a pathway that likely serves to limit cellular ROS production. This system centers on a potential electron acceptor that may compete with oxygen for electrons produced during cellular oxidative protein folding. We will focus on understanding the details of electron transfer within this system, as well as how disruption of this pathway negatively impacts cell physiology.
In Aim 2, we will describe the mechanistic details for a redox-signaling pathway we identified centered on a thiol-based redox switch in the molecular chaperone BiP. We will determine how activation of the BiP-thiol switch alters BiP activity and protects cells against oxidative damage. Successful completion of the proposed studies will provide insight into the basic cell functions used to manage cellular ROS and avert cellular damage.
Elevated levels of ROS are associated with the pathology of numerous degenerative disorders, including aging, Alzheimer's disease, diabetes, and atherosclerosis. A vast network of regulatory pathways exists within cells to manage intracellular ROS. Identification and characterization of these fundamental pathways in healthy cells is key to understanding what triggers the loss of ROS homeostasis associated with disease progression.
|Siegenthaler, Kevin D; Pareja, Kristeen A; Wang, Jie et al. (2017) An unexpected role for the yeast nucleotide exchange factor Sil1 as a reductant acting on the molecular chaperone BiP. Elife 6:|
|Xu, Mengni; Marsh, Heather M; Sevier, Carolyn S (2016) A Conserved Cysteine within the ATPase Domain of the Endoplasmic Reticulum Chaperone BiP is Necessary for a Complete Complement of BiP Activities. J Mol Biol 428:4168-4184|
|Brison, Yoann; Malbert, Yannick; Czaplicki, Georges et al. (2016) Structural Insights into the Carbohydrate Binding Ability of an ?-(1?2) Branching Sucrase from Glycoside Hydrolase Family 70. J Biol Chem 291:7527-40|
|Wang, Jie; Sevier, Carolyn S (2016) Formation and Reversibility of BiP Protein Cysteine Oxidation Facilitate Cell Survival during and post Oxidative Stress. J Biol Chem 291:7541-57|
|Wang, Jie; Pareja, Kristeen A; Kaiser, Chris A et al. (2014) Redox signaling via the molecular chaperone BiP protects cells against endoplasmic reticulum-derived oxidative stress. Elife 3:e03496|