In animals, including humans, mothers supply eggs with the mRNAs and proteins that drive the critical early stages of development. These maternally supplied factors directly control early developmental processes, and establish trajectories for the rest of development. Altering the levels of maternally deposited factors in model organisms in the laboratory can have dramatic effects on development, and hence adult phenotypes. Despite the importance of these maternally supplied factors to the development and fitness of the organism, little is known about how they evolve. This is of particular interest, because genetic control over this period of development has to be coordinated across two different genomes, that of the mother and that of the zygote, a form of epistasis which may pose a particular challenge to evolution. The fly species in the genus Drosophila, with its rich diversity of species covering 50 million years of divergence time, provide a great model for such a study. The Drosophila system has the advantage of extensive catalogs of the mRNAs and proteins deposited by females of the model species D. melanogaster, and much research has gone into understanding the specific developmental and regulatory roles of many of these factors. Despite the extent of the previous work in D. melanogaster, we know very little about how these maternally derived factors vary across species. This work proposes to: 1) characterize the mRNA pools in embryos, both those deposited by the mother and those later transcribed by the zygote, within and between Drosophila species, using mRNA sequencing methods, 2) determine phylogenetic patterns and life-history correlates of maternal and zygotic mRNA pool changes, and 3) identify genetic mechanisms underlying the evolutionary turnover of mRNA pools within and between species. The goal of this project is to understand how these mRNA pools evolve, and what the effect of these changes will be on the developmental process and the fitness of the organism.

Public Health Relevance

In animals, including humans, the critical early stages of development are driven almost entirely by gene products placed into the egg by the mother, which directly control early embryonic processes and establish trajectories for the rest of development. Despite the importance of these factors to the development and fitness of the organism, little is known about how they change over evolutionary time. This project will characterize changes in maternally deposited gene products over evolutionary time, and what the underlying genetic basis of these changes are so we can determine how they evolve.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Project (R01)
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Genetic Variation and Evolution Study Section (GVE)
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Janes, Daniel E
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University of California Davis
Schools of Arts and Sciences
United States
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Crofton, Amanda E; Cartwright, Emily L; Feitzinger, Anna A et al. (2018) Effect of Larval Nutrition on Maternal mRNA Contribution to the Drosophila Egg. G3 (Bethesda) 8:1933-1941
Paris, Mathilde; Villalta, Jacqueline E; Eisen, Michael B et al. (2015) Sex Bias and Maternal Contribution to Gene Expression Divergence in Drosophila Blastoderm Embryos. PLoS Genet 11:e1005592