Most bacterial pathogenesis studies have focused on mono-culture infections; however it is clear that many bacterial infections are not simply the result of colonization with a single species, but rather ensue from the action of polymicrobial communities. Microbes within polymicrobial infections often display synergistic interactions that result in enhanced colonization and persistence in the infection site. Such interactions have been particularly noted in wound infections, although the molecular processes controlling these synergistic interactions are generally not known. Detailed mechanistic studies of the polymicrobial interactions required for enhanced persistence in vivo are a necessary first step towards developing therapeutics to treat polymicrobial infections. The overall goal of this research plan is to determine how interactions between Pseudomonas aeruginosa and other microbes that commonly co-infect wounds impact wound severity. To accomplish this goal, in vivo murine wound models and high-throughput genomics techniques will be employed to identify and characterize microbial genes required for enhanced pathogenesis during co-infection.
Most bacterial infections are initiated by complex multi-species communities whose members work together to enhance disease symptoms, a phenomenon known as polymicrobial synergy. The objective of this research application is to provide novel insights into the mechanisms controlling synergy in the common human wound pathogen Pseudomonas aeruginosa during co-infection with other microbes. The ultimate goal of this research is to develop novel therapeutic strategies for treating polymicrobial infections.
|Smith, Allie Clinton; Rice, Anne; Sutton, Bryan et al. (2017) Albumin Inhibits Pseudomonas aeruginosa Quorum Sensing and Alters Polymicrobial Interactions. Infect Immun 85:|
|Ibberson, Carolyn B; Stacy, Apollo; Fleming, Derek et al. (2017) Co-infecting microorganisms dramatically alter pathogen gene essentiality during polymicrobial infection. Nat Microbiol 2:17079|
|Sønderholm, Majken; Kragh, Kasper Nørskov; Koren, Klaus et al. (2017) Pseudomonas aeruginosa Aggregate Formation in an Alginate Bead Model System Exhibits In Vivo-Like Characteristics. Appl Environ Microbiol 83:|
|Darch, Sophie E; Kragh, Kasper N; Abbott, Evelyn A et al. (2017) Phage Inhibit Pathogen Dissemination by Targeting Bacterial Migrants in a Chronic Infection Model. MBio 8:|
|Stacy, Apollo; McNally, Luke; Darch, Sophie E et al. (2016) The biogeography of polymicrobial infection. Nat Rev Microbiol 14:93-105|