Cancer cells and germ cells share many cellular and molecule features and the link is even more striking when considering the many tumors resulting from aberrant germ cells. Moreover, many tumors require germline determinants for tumorigenesis, even those tumors from a non- germ line origin. As one of the original germline determinants identified, Vasa have been used extensively as a germline marker in many organisms. Recently though Vasa has received more widespread attention for its'presence in many human cancer cells, its'essential nature in tumor formation, its'role in multipotent cell functions, and recently, it's'function in regulation of the cell cycle. Despite its'long history,the functions and regulation of Vasa in these important and widespread roles are not yet known. This project is designed to reveal the regulatory mechanisms of Vasa that contributes to its'essential function in health and disease.

Public Health Relevance

Vasa is a classic germ line marker now receiving widespread attention for it's'role in tumor formation, its'function in development of multipotent stem cells and its'essential function in the regulation of cell division. This project is designed to reveal he regulatory mechanisms of this essential protein in fundamental cellular processes of health and disease.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD028152-19
Application #
8438392
Study Section
Special Emphasis Panel (ZRG1-EMNR-E (02))
Program Officer
Ravindranath, Neelakanta
Project Start
1991-05-01
Project End
2017-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
19
Fiscal Year
2013
Total Cost
$308,707
Indirect Cost
$114,162
Name
Brown University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
001785542
City
Providence
State
RI
Country
United States
Zip Code
02912
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Oulhen, Nathalie; Wessel, Gary M (2014) Every which way--nanos gene regulation in echinoderms. Genesis 52:279-86
Swartz, S Zachary; Reich, Adrian M; Oulhen, Nathalie et al. (2014) Deadenylase depletion protects inherited mRNAs in primordial germ cells. Development 141:3134-42
Juliano, Celina; Swartz, S Zachary; Wessel, Gary (2014) Isolating specific embryonic cells of the sea urchin by FACS. Methods Mol Biol 1128:187-96
Campanale, Joseph P; Gökirmak, Tufan; Espinoza, Jose A et al. (2014) Migration of sea urchin primordial germ cells. Dev Dyn 243:917-27
Wessel, Gary M; Fresques, Tara; Kiyomoto, Masato et al. (2014) Origin and development of the germ line in sea stars. Genesis 52:367-77
Oulhen, Nathalie; Xu, Dongdong; Wessel, Gary M (2014) Conservation of sequence and function in fertilization of the cortical granule serine protease in echinoderms. Biochem Biophys Res Commun 450:1135-41
Oulhen, Nathalie; Onorato, Thomas M; Ramos, Isabela et al. (2014) Dysferlin is essential for endocytosis in the sea star oocyte. Dev Biol 388:94-102
Oulhen, Nathalie; Reich, Adrian; Wong, Julian L et al. (2013) Diversity in the fertilization envelopes of echinoderms. Evol Dev 15:28-40
Oulhen, Nathalie; Yoshida, Takaya; Yajima, Mamiko et al. (2013) The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin. Dev Biol 377:275-83

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