Genome maintenance in the germline is vital for fertility and the health of offspring. Although DNA damage response (DDR) and repair has been well characterized in meiocytes, the DDR pathway(s) that function in primordial germ cells (PGCs), which are the precursors to sperm and eggs, have not been identified. Fanconi anemia (FA) is a genomic instability syndrome associated with PGC depletion and infertility in addition to some other devastating clinical manifestations such as bone marrow failure and cancer predisposition. The FA pathway is a major DDR pathway known to function in double-strand DNA break repair. The signaling mechanisms underlying PGC depletion and infertility in FA patients or mouse models are not known. We recently developed Flag- and hemagglutinin-tagged Fancd2 knock-in mice that allowed a high throughput mass spectrometry approach to search for Fancd2-binding proteins in different mouse organs. In addition to DDR partners, we observed several proteins of the germ-cell-specific Prmt5/piRNA pathways orchestrating the repression of transposable elements (TEs). Deletion of Fancd2 resulted in decreased protein levels of the Prmt5/piRNA factors, massive upregulation of TEs, PGC depletion, and led to defective spermatogenesis and oogenesis in Fancd2-null (Fancd2-KO) mice. These preliminary studies suggest that in addition to its well-established DNA repair roles, Fancd2 and the FA pathway has an in vivo TE silencing function in early-stage germ cells. We hypothesize that the FA pathway is essential for germline integrity involving a pathway hierarchy in which the FA core signals to Fancd2, which then guides Prmt5 and piRNA in TE silencing. The goals of the project are to establish functional interaction between the FA pathway and germ cell- specific TE silencing machinery in safeguarding the germline genome, and to define Fancd2 as a crucial regulator of this vital epigenetic programming during germ cell development. The project presents an innovative study aimed at linking a major DDR pathway to germline genome maintenance in early-stage germ cells. The knowledge gained from the proposed study will not only improve mechanistic understanding of the molecular collaboration between the FA DDR pathway and the Prmt5/piRNA pathway in the context of TE repression, but also lead to a new avenue of research designed to target these interacting pathways for developing innovative therapeutic strategies for reproductive diseases such as infertility and birth defects.

Public Health Relevance

(Relevance Statement): The proposed project is relevant to public health because proper maintenance of the genetic material in the precursors to sperm and eggs is vital for fertility and the health of offspring. However, the mechanisms by which these precursor cells employ to prevent genetic damage remain poorly understood. This project aims to understand how the Fanconi anemia DNA repair pathway maintains genetic integrity in the precursors to sperm and eggs and how defect in that pathway can contribute to reproductive diseases such as infertility and birth defects.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD089932-02
Application #
9540723
Study Section
Development - 1 Study Section (DEV1)
Program Officer
Taymans, Susan
Project Start
2017-08-11
Project End
2021-03-31
Budget Start
2018-04-01
Budget End
2019-03-31
Support Year
2
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Cincinnati Children's Hospital Medical Center
Department
Type
DUNS #
071284913
City
Cincinnati
State
OH
Country
United States
Zip Code
45229
Du, Wei; Li, Xiaoli; Wilson, Andrew F et al. (2018) A small molecule p53 activator attenuates Fanconi anemia leukemic stem cell proliferation. Stem Cell Res Ther 9:145
Li, Xiaoli; Wilson, Andrew F; Du, Wei et al. (2018) Cell-Cycle-Specific Function of p53 in Fanconi Anemia Hematopoietic Stem and Progenitor Cell Proliferation. Stem Cell Reports 10:339-346
Abe, Hironori; Alavattam, Kris G; Kato, Yasuko et al. (2018) CHEK1 coordinates DNA damage signaling and meiotic progression in the male germline of mice. Hum Mol Genet 27:1136-1149
Du, Wei; Liu, Wei; Mizukawa, Benjamin et al. (2018) A non-myeloablative conditioning approach for long-term engraftment of human and mouse hematopoietic stem cells. Leukemia 32:2041-2046