We propose to complete the DNA sequence of the entire E. coli genome -- data gathering, assembly and analysis. In the course of this task we will continue research on and introduction of new techniques and strategies for sequencing. Management structures effective for this prototype large genome project will be developed. In addition we will develop and implement major innovative informatics programs to enhance sequence assembly and analysis, institute computerized data handling and storage, control the day-to-day sequencing program. A genomic database will be designed for use by E. coli scientists. This will also_contribute fundamental research on scientific databases containing many types of information with multiple relationships, and its accessibility to the complex inquiries of scientific users. Finally, we will establish an outreach interface with the scientific community.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
5R01HG000301-06
Application #
3333363
Study Section
Genome Research Review Committee (GRRC)
Project Start
1988-07-01
Project End
1995-06-30
Budget Start
1993-07-01
Budget End
1994-06-30
Support Year
6
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
Schools of Earth Sciences/Natur
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
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Sofia, H J; Burland, V; Daniels, D L et al. (1994) Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes. Nucleic Acids Res 22:2576-86
Blattner, F R; Burland, V; Plunkett 3rd, G et al. (1993) Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res 21:5408-17
Plunkett 3rd, G; Burland, V; Daniels, D L et al. (1993) Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res 21:3391-8
Burland, V; Daniels, D L; Plunkett 3rd, G et al. (1993) Genome sequencing on both strands: the Janus strategy. Nucleic Acids Res 21:3385-90
Burland, V; Plunkett 3rd, G; Daniels, D L et al. (1993) DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication. Genomics 16:551-61
Chuang, S E; Burland, V; Plunkett 3rd, G et al. (1993) Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli. Gene 134:1-6
Daniels, D L; Plunkett 3rd, G; Burland, V et al. (1992) Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science 257:771-8