Separation and analysis of restriction fragments by gel electrophoresis and Southern blotting are vital to the mapping of chromosomal DNA. At present, however, the information obtained from gels and blots is severely limited by the nonlinear response, limited dynamic range, low sensitivity and cumbersome processing inherent in the photographic methods used to record the distribution of fluorophore-stained or radionuclide-labeled DNA. This project will exploit recent advances in electronic imaging and computer technology that make possible accurate and rapid quantitation of the mass distribution of fluorophore-stained restriction fragments on gels and radionuclide-labeled probes on Southern blots. Computer programs for the storage and analysis of images of gels and blots will be developed to increase significantly the quality and quantity of information obtained while reducing the time required for data collection and processing.
| Mirsaidov, Utkur M; Wang, Deqiang; Timp, Winston et al. (2010) Molecular diagnostics for personal medicine using a nanopore. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2:367-81 |
| Dimitrov, V; Mirsaidov, U; Wang, D et al. (2010) Nanopores in solid-state membranes engineered for single molecule detection. Nanotechnology 21:065502 |
| Mirsaidov, Utkur; Comer, Jeffrey; Dimitrov, Valentin et al. (2010) Slowing the translocation of double-stranded DNA using a nanopore smaller than the double helix. Nanotechnology 21:395501 |
| Mirsaidov, U; Timp, W; Zou, X et al. (2009) Nanoelectromechanics of methylated DNA in a synthetic nanopore. Biophys J 96:L32-4 |
| Dorvel, B; Sigalov, G; Zhao, Q et al. (2009) Analyzing the forces binding a restriction endonuclease to DNA using a synthetic nanopore. Nucleic Acids Res 37:4170-9 |
| Zhao, Q; Comer, J; Dimitrov, V et al. (2008) Stretching and unzipping nucleic acid hairpins using a synthetic nanopore. Nucleic Acids Res 36:1532-41 |
| Cruz-Chu, Eduardo R; Aksimentiev, Aleksei; Schulten, Klaus (2006) Water-silica force field for simulating nanodevices. J Phys Chem B 110:21497-508 |
| Sutherland, J C; Monteleone, D C; Trunk, J G et al. (2001) Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis. Electrophoresis 22:843-54 |
| Sutherland, B M; Bennett, P V; Sutherland, J C (1999) DNA damage quantitation by alkaline gel electrophoresis. Methods Mol Biol 113:183-202 |
| Sutherland, J C; Monteleone, D C; Sutherland, B M (1997) Computer network for data acquisition, storage and analysis. J Photochem Photobiol B 40:14-22 |
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