Most cellular processes are carried out by multi-protein complexes. The identification and composition of such complexes is crucial for understanding how the ensemble of expressed proteins (The Proteome) is organized into functional units. The goal of the proposal is to establish a complete protein interaction map of the Drosophila melanogaster proteome based on the isolation and Mass spectrometric analysis of purified protein complexes associated with individually tagged proteins. This will determine the first complete metazoan proteome map beyond the level of binary protein interactions. The experimental system we propose to use offers an organism which has been extensively studied at a genetic and genomic level, has an established validity as a model for vertebrate biology, and offers the possibility of sophisticated genetic manipulations to determine cellular function. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
7R01HG003616-02
Application #
7263155
Study Section
Genomics, Computational Biology and Technology Study Section (GCAT)
Program Officer
Felsenfeld, Adam
Project Start
2007-04-16
Project End
2011-06-30
Budget Start
2007-04-16
Budget End
2007-06-30
Support Year
2
Fiscal Year
2006
Total Cost
$740,145
Indirect Cost
Name
Harvard University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115
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Sen, Anindya; Dimlich, Douglas N; Guruharsha, K G et al. (2013) Genetic circuitry of Survival motor neuron, the gene underlying spinal muscular atrophy. Proc Natl Acad Sci U S A 110:E2371-80
Guruharsha, K G; Kankel, Mark W; Artavanis-Tsakonas, Spyros (2012) The Notch signalling system: recent insights into the complexity of a conserved pathway. Nat Rev Genet 13:654-66
Guruharsha, K G; Obar, Robert A; Mintseris, Julian et al. (2012) Drosophila protein interaction map (DPiM): a paradigm for metazoan protein complex interactions. Fly (Austin) 6:246-53
Guruharsha, K G; Rual, Jean-Francois; Zhai, Bo et al. (2011) A protein complex network of Drosophila melanogaster. Cell 147:690-703
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Hurt, Jessica A; Obar, Robert A; Zhai, Bo et al. (2009) A conserved CCCH-type zinc finger protein regulates mRNA nuclear adenylation and export. J Cell Biol 185:265-77
Hurlbut, Gregory D; Kankel, Mark W; Artavanis-Tsakonas, Spyros (2009) Nodal points and complexity of Notch-Ras signal integration. Proc Natl Acad Sci U S A 106:2218-23

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