Abstract

of research plan. We will study the angiotensin I converting enzyme or kininase II purified from human lung. We will improve and simplify the currently employed purification procedures, elicit antibodies to the human enzyme and employ the antibody in radioimmunoassay. The activity of the enzyme will be studied in purified soluble form as well as in membrane-bound form. The hydrolysis of a variety of peptide substrates by the insoluble enzyme, will be established. Natural occurring inhibitors of the enzyme will be characterized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL036082-02
Application #
3350706
Study Section
Respiratory and Applied Physiology Study Section (RAP)
Project Start
1985-09-01
Project End
1989-08-31
Budget Start
1987-03-31
Budget End
1989-08-31
Support Year
2
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Illinois at Chicago
Department
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612

  Publications

Dragovic, T; Sekosan, M; Becker, R P et al. (1997) Detection of neutral endopeptidase 24.11 (neprilysin) in human hepatocellular carcinomas by immunocytochemistry. Anticancer Res 17:3233-8
Dragovic, T; Minshall, R; Jackman, H L et al. (1996) Kininase II-type enzymes. Their putative role in muscle energy metabolism. Diabetes 45 Suppl 1:S34-7
Jackman, H L; Tan, F; Schraufnagel, D et al. (1995) Plasma membrane-bound and lysosomal peptidases in human alveolar macrophages. Am J Respir Cell Mol Biol 13:196-204
Dragovic, T; Schraufnagel, D E; Becker, R P et al. (1995) Carboxypeptidase M activity is increased in bronchoalveolar lavage in human lung disease. Am J Respir Crit Care Med 152:760-4
Skidgel, R A (1995) Human carboxypeptidase N: lysine carboxypeptidase. Methods Enzymol 248:653-63
Tan, F; Deddish, P A; Skidgel, R A (1995) Human carboxypeptidase M. Methods Enzymol 248:663-75
Deddish, P A; Wang, J; Michel, B et al. (1994) Naturally occurring active N-domain of human angiotensin I-converting enzyme. Proc Natl Acad Sci U S A 91:7807-11
Minshall, R D; Yelamanchi, V P; Djokovic, A et al. (1994) Importance of sympathetic innervation in the positive inotropic effects of bradykinin and ramiprilat. Circ Res 74:441-7
Dragovic, T; Deddish, P A; Tan, F et al. (1994) Increased expression of neprilysin (neutral endopeptidase 24.11) in rat and human hepatocellular carcinomas. Lab Invest 70:107-13
Davies, P F; Robotewskyj, A; Griem, M L (1993) Endothelial cell adhesion in real time. Measurements in vitro by tandem scanning confocal image analysis. J Clin Invest 91:2640-52

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