The specific subject of this proposal is the regulatory regions of the gata-6 gene. The potential significance of this subject lies in the specificity of expression of this gene in the developing heart. This specificity appears to stem from control regions identified by the investigator in his preliminary studies. In particular, one reporter transgene containing the smallest fragment of control region was strikingly expressed only in the myocardium of the atrioventricular junction. The atrioventricular region is interesting area where mesenchymal transformation occurs, where connective tissue deposition is very important in later development for proper insulation of atrial from ventricular myocardium, and where valves develop. Defects in this region have been documented in many cardiovascular-associated diseases including Down Syndrome.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL055373-02
Application #
2750513
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Project Start
1997-08-15
Project End
2001-07-31
Budget Start
1998-08-01
Budget End
1999-07-31
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Institute for Cancer Research
Department
Type
DUNS #
872612445
City
Philadelphia
State
PA
Country
United States
Zip Code
19111
Wessels, Andy; van den Hoff, Maurice J B; Adamo, Richard F et al. (2012) Epicardially derived fibroblasts preferentially contribute to the parietal leaflets of the atrioventricular valves in the murine heart. Dev Biol 366:111-24
Eisenberg, Carol A; Burch, John B E; Eisenberg, Leonard M (2006) Bone marrow cells transdifferentiate to cardiomyocytes when introduced into the embryonic heart. Stem Cells 24:1236-45
Wilm, Bettina; Ipenberg, Annemieke; Hastie, Nicholas D et al. (2005) The serosal mesothelium is a major source of smooth muscle cells of the gut vasculature. Development 132:5317-28
Burch, John B E (2005) Regulation of GATA gene expression during vertebrate development. Semin Cell Dev Biol 16:71-81
Gaussin, Vinciane; Morley, Gregory E; Cox, Luk et al. (2005) Alk3/Bmpr1a receptor is required for development of the atrioventricular canal into valves and annulus fibrosus. Circ Res 97:219-26
Christoffels, Vincent M; Burch, John B E; Moorman, Antoon F M (2004) Architectural plan for the heart: early patterning and delineation of the chambers and the nodes. Trends Cardiovasc Med 14:301-7
Adamo, Richard F; Guay, Catherine L; Edwards, Angela V et al. (2004) GATA-6 gene enhancer contains nested regulatory modules for primary myocardium and the embedded nascent atrioventricular conduction system. Anat Rec A Discov Mol Cell Evol Biol 280:1062-71
He, C Z; Burch, J B (1997) The chicken GATA-6 locus contains multiple control regions that confer distinct patterns of heart region-specific expression in transgenic mouse embryos. J Biol Chem 272:28550-6