The capacity of the ventricles to perform work (i.e., power) is essential for moving blood throughout the circulatory system. Ventricular/myocyte power is determined ultimately by three myofibrillar properties;(i) the amount of force developed, (ii) the rate of force development, and (iii) the rate that myocardium shortens against loads. However, the sub-cellular processes that determine these properties are incompletely understood. The overall purpose of this study is to determine the sub-cellular factors that regulate myocyte power generating capacity. The specific objectives of the proposed studies are designed to determine (1) if PKA-mediated phosphorylation of cardiac troponin I (cTnI) is both necessary and sufficient to dictate sarcomere length dependence of force generation in cardiac myocytes, (2) how titin, MyBP-C, and myosin cross-bridges interact to modulate sarcomere length dependence of power output, and (3) to determine the time-course of changes in sarcomere length dependence of force, rates of force development, loaded shortening, and power output during the progression of heart failure. A rodent model of heart failure will be used to test the hypothesis that myocytes in compensated hearts exhibit greater sarcomere length dependence of force and power output due to a combination of greater expression of the larger N2BA titin isoform and greater PKA- induced myofibrillar protein phosphorylation, but with progression to ventricular failure sarcomere length dependence of force and power is markedly reduced due to reduced PKA- mediated phosphorylation of titin, MyBP-C, and cTnI. These studies are highly significant in their potential to determine the basis for the control of power-generating capacity in healthy myocytes and how these processes are altered with the progression of heart failure.

Public Health Relevance

The goal of this project is to understand the factors that control the power generating capacity of individual cardiac myocytes. The control of myocyte power output dictates the pumping capacity of healthy hearts and dysregulation of these control factors contributes to pathological heart conditions, which afflict nearly 6 million citizens of th United States and is the most common cause of hospitalization of US citizens over 65 years of age.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL057852-16
Application #
8626247
Study Section
Special Emphasis Panel (ZRG1-CVRS-B (02))
Program Officer
Adhikari, Bishow B
Project Start
1997-09-01
Project End
2016-02-28
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
16
Fiscal Year
2014
Total Cost
$363,289
Indirect Cost
$118,289
Name
University of Missouri-Columbia
Department
Pharmacology
Type
Schools of Medicine
DUNS #
153890272
City
Columbia
State
MO
Country
United States
Zip Code
65211
Hanft, Laurin M; Cornell, Timothy D; McDonald, Colin A et al. (2016) Molecule specific effects of PKA-mediated phosphorylation on rat isolated heart and cardiac myofibrillar function. Arch Biochem Biophys 601:22-31
Nance, Michael E; Whitfield, Justin T; Zhu, Yi et al. (2015) Attenuated sarcomere lengthening of the aged murine left ventricle observed using two-photon fluorescence microscopy. Am J Physiol Heart Circ Physiol 309:H918-25
Hanft, Laurin M; Greaser, Marion L; McDonald, Kerry S (2014) Titin-mediated control of cardiac myofibrillar function. Arch Biochem Biophys 552-553:83-91
Domeier, Timothy L; Roberts, Cale J; Gibson, Anne K et al. (2014) Dantrolene suppresses spontaneous Ca2+ release without altering excitation-contraction coupling in cardiomyocytes of aged mice. Am J Physiol Heart Circ Physiol 307:H818-29
Marshall, Kurt D; Muller, Brittany N; Krenz, Maike et al. (2013) Heart failure with preserved ejection fraction: chronic low-intensity interval exercise training preserves myocardial O2 balance and diastolic function. J Appl Physiol (1985) 114:131-47
Hanft, Laurin M; Biesiadecki, Brandon J; McDonald, Kerry S (2013) Length dependence of striated muscle force generation is controlled by phosphorylation of cTnI at serines 23/24. J Physiol 591:4535-47
Hinken, Aaron C; Hanft, Laurin M; Scruggs, Sarah B et al. (2012) Protein kinase C depresses cardiac myocyte power output and attenuates myofilament responses induced by protein kinase A. J Muscle Res Cell Motil 33:439-48
McDonald, Kerry S; Hanft, Laurin M; Domeier, Timothy L et al. (2012) Length and PKA Dependence of Force Generation and Loaded Shortening in Porcine Cardiac Myocytes. Biochem Res Int 2012:371415
Hanft, Laurin M; McDonald, Kerry S (2010) Length dependence of force generation exhibit similarities between rat cardiac myocytes and skeletal muscle fibres. J Physiol 588:2891-903
Hanft, Laurin M; McDonald, Kerry S (2009) Sarcomere length dependence of power output is increased after PKA treatment in rat cardiac myocytes. Am J Physiol Heart Circ Physiol 296:H1524-31

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