Evidence is building from epidemiological and laboratory investigations to support the hypothesis that genetic variation can modulate the effect of dietary intake on metabolic parameters to promote atherosclerosis and increase the incidence of coronary heart disease (CHD). Technological advances in molecular and nutritional epidemiology now make it possible to study gene-diet interactions and CHD in human populations at a new level of sophistication. The overall goal of this project is to carry out a population-based case-control study in 2,150 cases of myocardial infarction and 2,150 matched controls from Costa Rica, to test specific hypotheses relating gene-diet induced atherosclerosis susceptibility (GDAS) markers to CHD. Twelve GDAS markers were selected for this study. GDAS markers are defined as common genetic variants that modulate the effect of intake of specific fatty acids, tocopherols, and carotenoids on atherosclerosis. We will determine whether carriers of the GDAS marker variants are at increased risk of CHD compared to wild type homozygotes when exposed to high intakes of lauric 12:0, myristic 14:0, and palmitic 16:0, and trans fatty acids particularly 18:2 trans from partially hydrogenated soybean oil. We will study whether high intakes of alpha-linolenic acid, vitamin E, carotene, particularly alpha- carotene, lutein, and lycopene reduce the risk of CHD, and whether the GDAS marker variants alleles lessen this protective effect. In secondary analyses, we will test the hypotheses that the GDAS variant alleles influence the effect of dietary fiber, cholesterol, physical activity, and smoking on CHD. Haplotypes of metabolically related GDAS markers that are better predictors of CHD than individual markers alone will be established, and for each haplotype, we will determine specific adverse dietary patterns. Dietary exposure variables will be evaluated by simultaneous analyses of a semi-quantitative food frequency questionnaire, and biochemical measures of intake including adipose tissue tocopherols and carotenoids by HPLC, and fatty acids, including trans isomers of partially hydrogenated soybean oil by GC. This study will provide the most complete data set to study numerous hypotheses relating genes, diet, and CHD, and could lead to specific targeted interventions for reducing the development of CHD.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL060692-03
Application #
6389982
Study Section
Epidemiology and Disease Control Subcommittee 2 (EDC)
Program Officer
Sorlie, Paul
Project Start
1999-08-01
Project End
2004-07-31
Budget Start
2001-08-01
Budget End
2002-07-31
Support Year
3
Fiscal Year
2001
Total Cost
$1,158,214
Indirect Cost
Name
Harvard University
Department
Nutrition
Type
Schools of Public Health
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02115
Luan, D; Wang, D; Campos, H et al. (2018) Adipose tissue palmitoleic acid is inversely associated with nonfatal acute myocardial infarction in Costa Rican adults. Nutr Metab Cardiovasc Dis 28:973-979
Vadiveloo, Maya; Mattei, Josiemer (2017) Perceived Weight Discrimination and 10-Year Risk of Allostatic Load Among US Adults. Ann Behav Med 51:94-104
Sotos-Prieto, Mercedes; Baylin, Ana; Campos, Hannia et al. (2016) Lifestyle Cardiovascular Risk Score, Genetic Risk Score, and Myocardial Infarction in Hispanic/Latino Adults Living in Costa Rica. J Am Heart Assoc 5:
Vadiveloo, Maya K; Campos, Hannia; Mattei, Josiemer (2016) Seasoning ingredient variety, but not quality, is associated with greater intake of beans and rice among urban Costa Rican adults. Nutr Res 36:780-8
Cespedes, E; Baylin, A; Campos, H (2015) Adipose tissue n-3 fatty acids and metabolic syndrome. Eur J Clin Nutr 69:114-20
Toledo, Estefania; Campos, Hannia; Ding, Eric L et al. (2013) A novel fatty acid profile index--the lipophilic index--and risk of myocardial infarction. Am J Epidemiol 178:392-400
Gong, Jian; Campos, Hannia; Fiecas, Joseph Mark A et al. (2013) A case-control study of physical activity patterns and risk of non-fatal myocardial infarction. BMC Public Health 13:122
Mattei, Josiemer; Malik, Vasanti; Hu, Frank B et al. (2012) Substituting homemade fruit juice for sugar-sweetened beverages is associated with lower odds of metabolic syndrome among Hispanic adults. J Nutr 142:1081-7
Hartiala, Jaana; Gilliam, Elizabeth; Vikman, Susanna et al. (2012) Association of PLA2G4A with myocardial infarction is modulated by dietary PUFAs. Am J Clin Nutr 95:959-65
Castro-Webb, Nelsy; Ruiz-Narvaez, Edward A; Campos, Hannia (2012) Cross-sectional study of conjugated linoleic acid in adipose tissue and risk of diabetes. Am J Clin Nutr 96:175-81

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