Recent work suggests that mitochondria dysfunction plays a central role in sepsis, a major cause of death and morbidity in the United States. The underlying mechanisms responsible for this mitochondria dysfunction are not known. The goal of the present proposal is to test the hypothesis that increased free radical generation in sepsis produces specific biochemical, structural and genetic changes that result in marked physiologic alterations in mitochondrial function. We postulate: (a) mitochondria dysfunction in sepsis results from physiologic derangements of Krebs cycle enzymes, Complex I-IV electron transport chain components, and sarcomericcreatine kinase, (b) these physiologic changes are due, in turn, to alterations in the content and composition of mitochondrial proteins, and (c) protein changes are due, in part, to free radical-mediated decrements in mitochondrial gene transcription, expression, and translation. These hypotheses will be tested in three groups of experiments, using a model of endotoxin-induced sepsis. The purpose of Objective 1 is to fully characterize the specific physiologic derangements in the mitochondria in sepsis; we will examine Krebs cycle enzyme activities, evaluate specific performance of complexes within the electron transport chain, assess sarcomeric mitochondrial creatine kinase activity, and perform a metabolic control analysis. Objective II will identify changes in the content and composition of mitochondrial protein constituents (i.e. electron transport chain protein subunits, Krebs cycle enzymes, creatine kinase) and compare the time course of these alterations with the development of physiologic abnormalities determined in Objective I. Objective III will evaluate transcription, expression, and translation of mitochondria and nuclear genes encoding for mitochondrial proteins found to be depleted in Objective II. In all studies, we will determine the role of free radical modulation of these sepsis-induced changes. Our preliminary data provide the first evidence of substantial sepsis-associated oxidative modification and depletion of mitochondria protein subunits in Complexes I, III and IV, significant alterations in NADH generation via Krebs cycle enzymes, major decreases in mitochondria creatine kinase activity, and key free radical-mediated changes in gene expression of mitochondrial proteins in sepsis. These data suggest that the proposed experiments should provide important information regarding the pathogenesis of mitochondrial dysfunction in sepsis.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
7R01HL069821-06
Application #
7365401
Study Section
Respiratory and Applied Physiology Study Section (RAP)
Program Officer
Harabin, Andrea L
Project Start
2001-06-01
Project End
2008-05-31
Budget Start
2007-03-22
Budget End
2008-05-31
Support Year
6
Fiscal Year
2004
Total Cost
$227,232
Indirect Cost
Name
University of Kentucky
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
939017877
City
Lexington
State
KY
Country
United States
Zip Code
40506
Supinski, Gerald S; Callahan, Leigh Ann (2010) Calpain activation contributes to endotoxin-induced diaphragmatic dysfunction. Am J Respir Cell Mol Biol 42:80-7
Supinski, G S; Vanags, J; Callahan, L A (2009) Effect of proteasome inhibitors on endotoxin-induced diaphragm dysfunction. Am J Physiol Lung Cell Mol Physiol 296:L994-L1001
Supinski, G S; Murphy, M P; Callahan, L A (2009) MitoQ administration prevents endotoxin-induced cardiac dysfunction. Am J Physiol Regul Integr Comp Physiol 297:R1095-102
Callahan, Leigh Ann; Supinski, Gerald S (2009) Sepsis-induced myopathy. Crit Care Med 37:S354-67
Supinski, Gerald S; Ji, Xinying; Callahan, Leigh Ann (2009) The JNK MAP kinase pathway contributes to the development of endotoxin-induced diaphragm caspase activation. Am J Physiol Regul Integr Comp Physiol 297:R825-34
Callahan, Leigh Ann; Supinski, Gerald S (2005) Downregulation of diaphragm electron transport chain and glycolytic enzyme gene expression in sepsis. J Appl Physiol 99:1120-6
Supinski, Gerald S; Callahan, Leigh A (2005) Diaphragmatic free radical generation increases in an animal model of heart failure. J Appl Physiol 99:1078-84