Heart failure is a prevailing disease without effective treatment and represents a significant unmet medical need in the US. In response to pathological stresses, heart undergoes profound remodeling at molecular, cellular and organ levels. In previous studies, we and others have established that a stress-activated protein kinase, p38 plays a significant role in mediating pathological changes in heart under stress. We have demonstrated that constitutive activation of p38 in hearts leads to loss of contractility and pathological remodeling associated with pro- inflammatory cytokine induction. However, we have also observed that genetic inactivation of p38 leads to impaired survival and dysfunction under chronic stress or ageing. This paradox indicates complex roles for p38 mediated signaling in both deleterious and protective mechanisms in heart which has significant implications in the development of p38 targeted therapy for heart failure. To better understand the underlying mechanisms of p38 mediated signaling in heart, we have performed extensive studies at molecular, cellular and functional levels about p38 signaling complex and p38 mediated function. In particular, we have established that the auto-phosphorylation induced non-canonical p38 pathway is regulated by a novel interacting partner, Hsp90/Cdc37 complex. We have also discovered that p38 activity is critical to compensatory vascular remodeling in heart via paracrine cross-talk from cardiomyocytes to endothelial cells. Finally, we have demonstrated that a well established p38 downstream kinase MK2 has a selective contribution to p38 induced pathological changes in heart. These novel findings lead to our current hypothesis that diverse mechanisms in p38 activation and downstream targets contribute to specific roles of p38 signaling in both compensatory and pathological remodeling in heart. In the current proposal, we plan to advance our current knowledge of p38 mediated stress signaling by accomplishing the following specific aims: 1). Determine the molecular mechanism and the functional significance of non-canonical p38 kinase activation in heart. 2). Characterize the mechanisms underlying p38 mediated regulation of cardiomyocyte and endothelium cross-talk during pathological remodeling of heart. 3). Uncover the functional significance of downstream kinase MK2 in p38 mediated stress-response in heart. These studies will significantly advance our current knowledge in the disease mechanisms of heart failure and help to develop more effective therapy for the disease.

Public Health Relevance

Stress kinase p38 is an important signaling pathway in stress-response. Our proposal will investigate the molecular mechanisms and functional role of a novel non-canonical activation pathway of p38 and its impact on crosstalk from myocyte to endothelial cells. These studies will improve our current understanding to the disease mechanisms of heart failure and provide potential insights for development of better treatment.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL103205-03
Application #
8235829
Study Section
Cardiac Contractility, Hypertrophy, and Failure Study Section (CCHF)
Program Officer
Wong, Renee P
Project Start
2010-04-02
Project End
2015-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
3
Fiscal Year
2012
Total Cost
$381,150
Indirect Cost
$133,650
Name
University of California Los Angeles
Department
Anesthesiology
Type
Schools of Medicine
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Sun, Haipeng; Wang, Yibin (2014) Interferon regulatory factors in heart: stress response beyond inflammation. Hypertension 63:663-4
Wang, Yibin (2014) Blind dates in sciences: dealing with rejection in peer review. Circ Res 114:944-6
Xiao, Xinshu Grace; Touma, Marlin; Wang, Yibin (2014) Decoding the noncoding transcripts in human heart failure. Circulation 129:958-60
Ahuja, Preeti; Wanagat, Jonathan; Wang, Zhihua et al. (2013) Divergent mitochondrial biogenesis responses in human cardiomyopathy. Circulation 127:1957-67
Foster, William H; Langenbacher, Adam; Gao, Chen et al. (2013) Nuclear phosphatase PPM1G in cellular survival and neural development. Dev Dyn 242:1101-9
Force, Thomas; Wang, Yibin (2013) Mechanism-based engineering against anthracycline cardiotoxicity. Circulation 128:98-100
Sun, Haipeng; Wang, Yibin (2012) Novel Ser/Thr protein phosphatases in cell death regulation. Physiology (Bethesda) 27:43-52
Marber, Michael S; Rose, Beth; Wang, Yibin (2011) The p38 mitogen-activated protein kinase pathway--a potential target for intervention in infarction, hypertrophy, and heart failure. J Mol Cell Cardiol 51:485-90
Sun, Haipeng; Wang, Yibin (2011) Restriction of big hearts by a small RNA. Circ Res 108:274-6
Huang, Ying; Zhou, Meiyi; Sun, Haipeng et al. (2011) Branched-chain amino acid metabolism in heart disease: an epiphenomenon or a real culprit? Cardiovasc Res 90:220-3

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