This proposal describes a 5-year research plan focusing on how signal transduction through the T cell receptor (TCR ) contributes to the homeostasis and expansion of regulatory T cells (Treg)s. Tregs are a subset of T cells with suppressive function and are crucial for preventing immunopathology by inhibiting conventional T cell (Tconv) activation. A failure to develop Tregs or to maintain Tregs in the periphery results in fatal autoimmune disease. In contrast, an increase in Tregs protects against a variety of T cell-mediated disorders including graft-versus-host disease (GVHD). Thus, understanding the mechanisms by which Tregs proliferate and are maintained is of critical importance. However, the precise cellular and molecular requirements for Treg homeostasis and expansion are incompletely understood. We have recently repored that Tconvs and dendritic cells (DC)s play a critical role in controlling Treg homeostasis and expansion. Our preliminary data suggest that (1) Tregs require DCs but not TCR stimulation for their proliferation, (2) TCR stimulation of Tconvs by DCs for IL-2 production is required for Treg proliferation, and (3) blockade of TCR stimulation in conjunction with exogenous IL-2 inhibits Tconv activation but maintains Treg proliferation. These data support our hypothesis that the homeostatic maintenance and proliferation of Tregs depend on Tconvs and DCs, and occur through both TCR/MHC II-dependent and - independent routes. We propose that TCR stimulation allows the preferential expansion of Tregs with relevant antigen specificities, while the MHC II/TCR-independent mode of Treg proliferation ensures survival of Tregs with TCRs of multiple specificities to continuously maintain a diverse repertoire of Tregs. In this proposal, we aim to extend our preliminary in vitro findings to in vivo studies. Our research plan starts by examining the precise contribution of TCR signaling by Tregs to their homeostasis in vivo.
Our second aim will test the role of TCR signaling by Tconvs for IL-2 production in supporting Treg homeostasis in vivo. Finally, we will translate our hypothesis to clinically relevant questions by exploiting the differential requirement of TCR signaling in Treg and Tconv proliferation and determine whether a combination of IL-2 and a TCR signaling inhibitor such as CSA could be used to more effectively treat Tconv-mediated diseases such as GVHD. The proposed studies will enhance our understanding of Treg homeostasis and potentially yield novel molecules, strategies, and pathways to modulate Tregs in multiple disease settings. Our expertise in signal transduction and Treg biology together with our expert consultants (Drs. Andy Caton, Gary Koretzky, Robert Eisenberg, and Andras Schaffer), and the quality of the research facilities at the University of Pennsylvania Medical Center comprise an ideal environment in which to conduct this proposed research plan.
Regulatory T cells play a critical role in the prevention of immunopathology and can be used to treat a variety of T cell-mediated diseases including autoimmunity and graft-versus-host disease. The goal of this proposal is to investigate the cellular and molecular requirements, in particular the role of dendritic cells and the T cell receptor, in regulatory T cell homeostasis and expansion. The proposed studies will enhance our understanding of regulatory T cell homeostasis and potentially yield novel molecules, strategies, and pathways to modulate regulatory T cells in multiple disease settings.
|Singh, Brenal K; Kambayashi, Taku (2016) The Immunomodulatory Functions of Diacylglycerol Kinase Î¶. Front Cell Dev Biol 4:96|
|Yang, Enjun; Singh, Brenal K; Paustian, Amanda M Schmidt et al. (2016) Diacylglycerol Kinase Î¶ Is a Target To Enhance NK Cell Function. J Immunol 197:934-41|
|Freund, Jacquelyn; May, Rebecca M; Yang, Enjun et al. (2016) Activating Receptor Signals Drive Receptor Diversity in Developing Natural Killer Cells. PLoS Biol 14:e1002526|
|Schmidt, Amanda M; Lu, Wen; Sindhava, Vishal J et al. (2015) Regulatory T cells require TCR signaling for their suppressive function. J Immunol 194:4362-70|
|Karimi, Mobin A; Bryson, Jerrod L; Richman, Lee P et al. (2015) NKG2D expression by CD8+ T cells contributes to GVHD and GVT effects in a murine model of allogeneic HSCT. Blood 125:3655-63|
|Reshef, Ran; Huffman, Austin P; Gao, Amy et al. (2015) High Graft CD8 Cell Dose Predicts Improved Survival and Enables Better Donor Selection in Allogeneic Stem-Cell Transplantation With Reduced-Intensity Conditioning. J Clin Oncol 33:2392-8|
|Arumugam, Vidhyalakshmi; Bluemn, Theresa; Wesley, Erin et al. (2015) TCR signaling intensity controls CD8+ T cell responsiveness to TGF-Î². J Leukoc Biol 98:703-12|
|Huang, Yanping; Clarke, Fiona; Karimi, Mobin et al. (2015) CRK proteins selectively regulate T cell migration into inflamed tissues. J Clin Invest 125:1019-32|
|Satake, Atsushi; Schmidt, Amanda M; Nomura, Shosaku et al. (2014) Inhibition of calcineurin abrogates while inhibition of mTOR promotes regulatory T cell expansion and graft-versus-host disease protection by IL-2 in allogeneic bone marrow transplantation. PLoS One 9:e92888|
|Karimi, Mobin A; Lee, Eric; Bachmann, Michael H et al. (2014) Measuring cytotoxicity by bioluminescence imaging outperforms the standard chromium-51 release assay. PLoS One 9:e89357|
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