Heart failure (HF) is a major cause of morbidity and mortality in the developed world, but the molecular mechanisms that lead to the development of HF are not totally understood. Mammalian Snf1-related kinase (SNRK) is a serine/threonine kinase with sequence similarity to AMP-activated kinases (AMPK); however, its primary function is unknown. To better define the function of SNRK in cardiac metabolism, we have generated a transgenic (TG) mice that overexpress SNRK in the heart. SNRK TG mice display 30- 50% less glucose and fatty acid (FA) oxidation and have reduced oxygen consumption despite maintaining normal cardiac function, suggesting that they are more efficient in substrate utilization. The mechanism for metabolic efficiency appears to be through improved mitochondrial coupling, as the levels of uncoupling protein (UCP)-3 are reduced in SNRK TG mice, and state 3/state 4 respiration is increased. To determine the mechanism by which SNRK regulates mitochondrial coupling, we performed a yeast two hybrid screen to identify novel binding partners of SNRK. Tribbles homolog 3 (Trib3), a negative regulator of Akt activation and glucose metabolism, was found to bind to and be upregulated by SNRK. We also showed that SNRK regulates UCP3 and mitochondrial coupling through a pathway that involves Trib3 and PPAR?. The central hypothesis of this proposal is that SNRK affects cardiac metabolism by reducing substrate utilization and increasing mitochondrial coupling, and that SNRK overexpression protects against the development of HF and ischemic injury.
In Aim 1, we will assess whether deletion of SNRK will result in the opposite phenotype of its overexpression, i.e., increased glucose and FA metabolism with similar cardiac force. We have generated global SNRK+/- mice and cardiac-specific SNRK knockout (KO) mice, and will measure cardiac metabolic parameters in these animals. We will also assess the role of Trib3, UCP3 and PPAR? in this process.
In Aim 2, we will test whether the reduced glucose and FA metabolism that occurs in the hearts of SNRK TG mice is mediated through Trib3. We have generated SNRK TG/Trib3 KO mice, and will measure glucose and FA metabolism in their hearts. We will also investigate the mechanism of Trib3 regulation by SNRK, and PPAR? regulation by Trib3. Finally, in Aim 3, we will determine whether SNRK is protective against the development of HF and ischemic damage, and will study the mechanism for this protective effect. We will subject SNRK TG mice to ischemia-reperfusion and pressure overload, followed by the measurement of cardiac function and reactive oxygen species production (using novel fluorescence probes). To determine the mechanism, we will assess the role of Trib3/PPAR?/UCP3 pathway in this process. Our studies showing that SNRK reduces FA and glucose metabolism and improves mitochondrial coupling promise to advance our knowledge of the role of cardiac metabolism in HF, and may lead to the development of novel therapies for this disorder.

Public Health Relevance

Heart cells alter their metabolism in response to changes in their environment, especially in the setting of heart failure and heart attack. In this proposal, w will determine whether a novel protein called sucrose non-fermenting-1 related kinase (SNRK) plays a role in regulating heart metabolism and its progression to heart failure in response to injury. We will study whether this protein makes the heart more metabolically efficient and will determine the mechanism for this process.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL127646-03
Application #
9392186
Study Section
Cardiac Contractility, Hypertrophy, and Failure Study Section (CCHF)
Program Officer
Wong, Renee P
Project Start
2015-12-16
Project End
2019-11-30
Budget Start
2017-12-01
Budget End
2018-11-30
Support Year
3
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Northwestern University at Chicago
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611
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