The main focus of this application is on the functions of G-proteins in endothelial cells. G-protein G?13 is critical for blood vessel formation. This proposal builds on our discovery of a novel mechanism by which G?13 functions through SOS, a direct regulator of Ras and the new observation that endothelial cell-specific deletion of G?13 impairs organ regeneration.
The first aim i s to dissect the biochemical mechanism by which G?13 regulates SOS in vitro.
The second aim i s to determine how G?13 and G-protein- coupled receptors regulate SOS in cells.
The third aim i s to investigate the impact of endothelial cell-specific deletion of SOS1 on organ regeneration.
This research is directly related to human health. Endothelial cells are essential for angiogenesis and vascular development. Therefore, a better understanding of the regulatory mechanisms of endothelial functions will advance our combat against many human diseases such as cardiovascular diseases and cancers.
|Wang, Limin; Wang, Dawei; Xing, Bowen et al. (2017) G-Protein G?13 Functions with Abl Kinase to Regulate Actin Cytoskeletal Reorganization. J Mol Biol 429:3836-3849|
|Syrovatkina, Viktoriya; Alegre, Kamela O; Dey, Raja et al. (2016) Regulation, Signaling, and Physiological Functions of G-Proteins. J Mol Biol 428:3850-68|