Depression and anxiety are prevalent mood disorders symptomatic of the syndrome described as "NeuroAIDS," and pose significant problems for the treatment and well-being of HIV-positive patients. Unfortunately, the biological mechanisms linking HIV-related neuropathology to the dysfunction of neuronal circuitry and the progression of behavioral mood disorders are not well understood. Recent evidence suggests that HIV-accessory proteins such as Tat may spread throughout the brain from HIV-infected cells, producing neurotoxicity and changes in neuronal activity that could account for the behavioral changes. We hypothesize that Tat protein expression is sufficient to produce neurodegeneration and dysfunction of neuronal circuitry mediating mood, resulting in an increase of depression- and anxiety-like behaviors. This proposal utilizes the GT-tg transgenic mouse and its doxycycline-gene induction strategy for a controlled, selective expression of Tat protein in the brain. Induced GT-tg bigenic mice will be used to test the hypothesis with behavioral and imaging studies that Tat-mediated increases in depression- and anxiety-like behaviors are correlated with Tat-induced alterations in brain structure and function in regions associated with depression and anxiety, including the amygdala, anterior cingulate, orbitofrontal cortex and hippocampus. Behavioral studies with these mice will assess how Tat expression affects depression-like behaviors with the forced swim stress and social aversion tests. Behavioral experiments also will examine effects of Tat expression on anxiety-like behaviors using the open field, elevated plus maze, light-dark box, acoustic startle and marble burying tests. Assays of Tat mRNA and protein levels will correlate expression of Tat to the observed changes in behavior. In preliminary studies, Tat-induced mice spent less time than uninduced littermates in social interactions and more time immobile in forced swim stress tests, suggestive of a Tat-mediated increase in depression-like behavior. Tat-induced mice also demonstrated increased anxiety-related behaviors, with less time spent in open-field environments and a 3-fold increase in marble burying. Concurrently, we will examine the effects of Tat protein on brain structure and cell death using ex vivo magnetic resonance imaging (MRI) at ultra high magnetic field strength and TUNEL staining for apoptosis in Tat-induced animals. Preliminary ex vivo structural imaging studies documented significant reductions in the grey matter density of amygdala and hippocampus in Tat-expressing mice. Additionally, we will use BOLD functional MRI with corticosterone challenge to identify functional changes in brain regions associated with mood disorders resulting from varying levels of induction and duration of exposure to Tat protein and the response to a challenge dose of corticosterone. Overall, this project seeks to prove that functional and structural deficits can be detected in the brain circuitry of Tat-induced animals, thereby defining mechanisms by which Tat may mediate the mood disorders characteristic of NeuroAIDS.
The prevalence of mood disorders in people with HIV is high, and this comorbidity increases morbidity and mortality. We hypothesize that mood disorders associated with HIV infection are mediated by the neuronal dysfunction and neurodegeneration induced by the HIV-accessory protein, Tat. We will test this theory using a transgenic mouse model enabling selective, controlled Tat protein expression in brain to study behaviors relevant to depression and anxiety along with brain structure and function, using magnetic resonance neuroimaging. The findings will begin to clarify whether Tat protein should be a target for new treatments that block or reduce HIV-related neuropathology and mood disorders.
|Mediouni, Sonia; Jablonski, Joseph; Paris, Jason J et al. (2015) Didehydro-cortistatin A inhibits HIV-1 Tat mediated neuroinflammation and prevents potentiation of cocaine reward in Tat transgenic mice. Curr HIV Res 13:64-79|
|Paris, Jason J; Singh, Harminder D; Carey, Amanda N et al. (2015) Exposure to HIV-1 Tat in brain impairs sensorimotor gating and activates microglia in limbic and extralimbic brain regions of male mice. Behav Brain Res 291:209-18|
|Carey, Amanda N; Liu, Xiaoxu; Mintzopoulos, Dionyssios et al. (2015) Conditional Tat protein brain expression in the GT-tg bigenic mouse induces cerebral fractional anisotropy abnormalities. Curr HIV Res 13:3-9|
|Paris, Jason J; Singh, Harminder D; Ganno, Michelle L et al. (2014) Anxiety-like behavior of mice produced by conditional central expression of the HIV-1 regulatory protein, Tat. Psychopharmacology (Berl) 231:2349-60|
|Paris, Jason J; Carey, Amanda N; Shay, Christopher F et al. (2014) Effects of conditional central expression of HIV-1 tat protein to potentiate cocaine-mediated psychostimulation and reward among male mice. Neuropsychopharmacology 39:380-8|
|Paris, Jason J; Fenwick, Jason; McLaughlin, Jay P (2014) Progesterone protects normative anxiety-like responding among ovariectomized female mice that conditionally express the HIV-1 regulatory protein, Tat, in the CNS. Horm Behav 65:445-53|
|Paris, Jason J; Fenwick, Jason; McLaughlin, Jay P (2014) Estrous cycle and HIV-1 Tat protein influence cocaine-conditioned place preference and induced locomotion of female mice. Curr HIV Res 12:388-96|
|McLaughlin, Jay P; Ganno, Michelle L; Eans, Shainnel O et al. (2014) HIV-1 Tat protein exposure potentiates ethanol reward and reinstates extinguished ethanol-conditioned place preference. Curr HIV Res 12:415-23|
|Carey, Amanda N; Liu, Xiaoxu; Mintzopoulos, Dionyssios et al. (2013) Conditional Tat protein expression in the GT-tg bigenic mouse brain induces gray matter density reductions. Prog Neuropsychopharmacol Biol Psychiatry 43:49-54|
|Paris, Jason J; Eans, Shainnel O; Mizrachi, Elisa et al. (2013) Central administration of angiotensin IV rapidly enhances novel object recognition among mice. Neuropharmacology 70:247-53|
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