The goal of this research proposal is to develop a strategy to use patient derived cells with defined disease genotypes to identify disrupted molecular pathways through large-scale proteomics and network analysis. We have named this approach proteogenetics. Human induced pluripotent stem cells (hiPSC) have revolutionized the ability to study human diseases from patients. It is now possible to obtain fibroblasts from patients suffering from a disease and to reprogram the cells to pluripotent stem cells and then differentiate them into a cell type associated with the disease state. This reverses a long standing limitation for the study of human diseases has been the ability to use cells directly from patients with the appropriate disease phenotype and genotype. To study Rett's syndrome, we will combine vertical (unaffected parent and affected child) and horizontal genetics (different mutations in families/patients) to measure proteomic changes in affected forebrain neuronal cells (FNC) derived through fibroblasts and hiPSCs. For schizophrenia, we will use cells from several patients, but will not have access to unaffected parents. We will use network analysis techniques to identify pathway based phenotypic differences from protein and phosphoprotein expression patterns. Fibroblasts have been obtained from unaffected parents and affected children for Rett's syndrome and schizophrenia patients by Professor Allyson Moutri's (UCSD) and Professor Fred Gage's laboratory (The Salk Institute), respectively. These cells have been reprogrammed to iPSCs and differentiated into forebrain neurons for proteomic analysis. Our hypothesis is that this approach will identify specific molecular processes disrupted by disease.

Public Health Relevance

The goal of this research proposal is to develop a strategy to use patient derived cells induced pluripotent stem cells with defined disease genotypes to identify disrupted molecular pathways through large-scale proteomics and network analysis. By using a combination of vertical and horizontal genetics to study how protein networks are affected by perturbations to the genetic programs of these cells, we will determine the biochemical implications of the patient genotypes. This research will drive our understanding of the pathways perturbed by the disease, creating a new focus for therapies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Project (R01)
Project #
1R01MH100175-01
Application #
8480065
Study Section
Enabling Bioanalytical and Imaging Technologies Study Section (EBIT)
Program Officer
Senthil, Geetha
Project Start
2013-08-01
Project End
2015-07-31
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
1
Fiscal Year
2013
Total Cost
$542,713
Indirect Cost
$227,021
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Brennand, K; Savas, J N; Kim, Y et al. (2015) Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia. Mol Psychiatry 20:361-8
Savas, Jeffrey N; De Wit, Joris; Comoletti, Davide et al. (2014) Ecto-Fc MS identifies ligand-receptor interactions through extracellular domain Fc fusion protein baits and shotgun proteomic analysis. Nat Protoc 9:2061-74
Lavallée-Adam, Mathieu; Rauniyar, Navin; McClatchy, Daniel B et al. (2014) PSEA-Quant: a protein set enrichment analysis on label-free and label-based protein quantification data. J Proteome Res 13:5496-509