The field of human genetics has been transformed by genome-wide association studies (GWAS), which have identified thousands of genomic loci that influence hundreds of diseases and traits. In order to interpret the mechanisms by which these loci act, consortia and individual investigators have generated hundreds of functional genomic data sets with the goal of identifying important gene regulatory elements. This proposal describes a set of novel methods for combining hundreds of functional genomics datasets with a large GWAS of schizophrenia to identify the precise cell types, pathways, and gene regulatory elements most relevant to disease.
In Aim 1, we describe a statistical method for fine-mapping risk loci using functional genomic information. We propose to systematically scan through thousands of genomic annotations to identify those that are most important for understanding schizophrenia risk.
In Aim 2, we propose to combine GWAS data with gene expression data by jointly analyzing association studies of traits with association studies of gene expression (expression quantitative trait loci). Finally, in Aim 3, we propose to extend these methods to analyze multiple GWAS at once, with a specific emphasis on inferring the causal relationships between traits. The methods developed in this proposal will be widely application in studies of disease, and the analyses performed will generate direct predictions about the molecular consequences of alleles that influence schizophrenia risk.

Public Health Relevance

In the last several years, many genomic regions that influence schizophrenia risk have been identified. However, the individual genetic variants that cause disease risk, and the biological mechanisms by which they do so, remain unclear. This proposal describes a set of statistical tools for addressing these issues.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Project (R01)
Project #
5R01MH106842-03
Application #
9225225
Study Section
Special Emphasis Panel (ZRG1-IMST-D (55)R)
Program Officer
Arguello, Alexander
Project Start
2015-04-01
Project End
2018-02-28
Budget Start
2017-03-01
Budget End
2018-02-28
Support Year
3
Fiscal Year
2017
Total Cost
$408,919
Indirect Cost
$171,220
Name
New York Genome Center
Department
Type
Research Institutes
DUNS #
078473711
City
New York
State
NY
Country
United States
Zip Code
10013
Mohammadi, Pejman; Castel, Stephane E; Brown, Andrew A et al. (2017) Quantifying the regulatory effect size of cis-acting genetic variation using allelic fold change. Genome Res 27:1872-1884
Kim-Hellmuth, Sarah; Bechheim, Matthias; Pütz, Benno et al. (2017) Genetic regulatory effects modified by immune activation contribute to autoimmune disease associations. Nat Commun 8:266
Kim-Hellmuth, Sarah; Lappalainen, Tuuli (2016) Concerted Genetic Function in Blood Traits. Cell 167:1167-1169
Berisa, Tomaz; Pickrell, Joseph K (2016) Approximately independent linkage disequilibrium blocks in human populations. Bioinformatics 32:283-5
Pickrell, Joseph K; Berisa, Tomaz; Liu, Jimmy Z et al. (2016) Detection and interpretation of shared genetic influences on 42 human traits. Nat Genet 48:709-17